Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.21 (
CPT
)
4,580
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Human Topors has originally been identified as binding partner of p53 and DNA topoisomerase I (TOP1). It can function as both an ubiquitin and
SUMO-1
E3 ligase for p53. Here we demonstrate that Topors enhances the formation of high-molecular weight
SUMO-1
conjugates of TOP1 in a reconstituted in vitro system and also in human osteosarcoma cells, similar to treatment with
CPT
. In contrast to the situation observed with p53, overall sumoylation levels were rather unaffected. Experiments with TOP1 point mutants strongly suggest that the high-molecular weight conjugates represent
SUMO-1
chains formed on a limited number of
SUMO-1
acceptor sites.
...
PMID:The E3 ligase Topors induces the accumulation of polysumoylated forms of DNA topoisomerase I in vitro and in vivo. 1797 81
