Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.3.1.21 (CPT)
 4,580 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In order to explore the range of biological activities of the camptothecin compound class, the in vitro antiviral efficacies of series I-IV of representative members from camptothecin analogues on herpes simplex virus type 2 (HSV-2) were evaluated on vero cells. Several compounds exhibited similar or better antiviral activity against HSV-2 in vitro than acyclovir. Among them, compound 6 showed the highest anti-HSV-2 activity, with IC(50) values of 1.3 microg mL(-1) and SI values of 27.04, respectively. On the basis of preliminary biological testing results, it is suggested that the intact E lactone ring and 20-hydroxy group should be not a prerequisite for camptothecin-like antiviral activity, and CPT substitution at the C-20 hydroxy group may be optimal for synthesising more potent antiviral compounds, suggesting that CPT has the potential to be a lead structure for semi-synthetic antiviral agents.
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PMID:Anti-HSV activity of camptothecin analogues. 2039 3

We have developed a rapid and simple molecular test, the IsoGlow HSV Typing assay, for the detection and typing of herpes simplex virus (type 1 and 2) from genital or oral lesions. Clinical samples suspended in viral transport mediums are simply diluted and then added to a helicase-dependent amplification master mix. The amplification and detection were performed on a portable fluorescence detector called the FireFly instrument. Detection of amplification products is based on end-point analysis using cycling probe technology. An internal control nucleic acid was included in the amplification master mix to monitor the presence of amplification inhibitors in the samples. Because the device has only two fluorescence detection channels, two strategies were developed and compared to detect the internal control template: internal control detected by melting curve analysis using a dual-labeled probe, versus internal control detection using end-point fluorescence release by a CPT probe at a lower temperature. Both have a total turnaround time of about 1 hour. Clinical performance relative to herpes viral culture was evaluated using 176 clinical specimens. Both formats of the IsoGlow HSV typing assay had sensitivities comparable to that of the Food and Drug Administration-cleared IsoAmp HSV (BioHelix Corp., Beverly MA) test and specificity for the two types of HSV comparable to that of ELVIS HSV (Diagnostic Hybrids, Athens, OH).
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PMID:Development and comparison of a rapid isothermal nucleic acid amplification test for typing of herpes simplex virus types 1 and 2 on a portable fluorescence detector. 2295 87