Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.3.1.184 (LasR)
 897 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A new technique by which sperm antibodies are purified by affinity-chromatography in a batch device and the sperm agglutinating activity controlled by the Franklin and Dukes (FD) test is described. Specific absorption takes place on an insoluble antigen and desorption reveals purified antibodies. In sera of women with unexplained infertility which were found to be positive to the FD test a specific globulin binding takes place. For quantitative detection of these purified antibodies, crossed immunoelectrophoresis was done. Sera of 4 such infertile women were used. Uterine cervical mucus samples of 3 of them were also examined. As controls, sera negative to the FD test and cervical mucus from the same patients were processed. Spermatozoa were obtained from 15 donors, pooled, washed 3 times with phosphate-buffered saline, and adjusted to spermatozoa content. Cervical mucus was also prepared. Techniques used are given. The method can be easily and quickly performed and therefore used routinely. Antibody fractions, purified by affinity-chromatography migrating against an AHS-containing gel, demonstrated 1 big peak in all 4 of the FD-positive semen samples as well as in the 3 cervical mucus samples. The height of the peak represented a nearly linear relation to the sperm antibody content of the semen. Control samples never showed this peak. By absorption experiments it was found that the protein encountered in serum is IgM and that in cervical mucus IgA. The content of antibodies in the cervical mucus of the same woman was about 1/10 smaller than in her serum. This method is stated to be an improvement over other techniques. Some sperm antibodies are not of the agglutinating type.
 ...
PMID:Crossed immunoelectrophoresis of sperm antibodies in human serum and cervical mucus. 0 87

Clearance of aggregated human secretory immunoglobulin A (AHS-IgA) was studied in nine patients affected by primary IgA nephropathy (IgAGN) and six normal volunteers from the medical staff. Samples of whole blood, erythrocytes, and serum were taken from 3 to 120 minutes after injection of 0.5 mg of 131iodine-labeled AHS-IgA. No significant radioactivity was recorded on erythrocytes. The clearance curve of AHS-IgA from the circulation, calculated by measuring trichloroacetic acid precipitable radioactivity in serum, was found to be biexponential with an initial fast component significantly prolonged in patients (mean half-time, 19.4 minutes, range, 14 to 26 minutes) compared with controls (mean, 12.2 minutes, range, 7.6 to 16.8 minutes; P less than 0.01). These data indicate that clearance of aggregated polymeric IgA does not involve the erythrocyte transport system and seems to be defective in IgAGN patients.
 ...
PMID:Clearance of polymeric IgA aggregates in humans. 281 27