Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.184 (
LasR
)
897
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Two almost identical gene clusters (
phz1
and
phz2
) are responsible for phenazine-1-carboxylic acid (PCA) production in
Pseudomonas aeruginosa
(
P
.
aeruginosa
) strain
MSH
(derived from strain PA1201). Here, we showed that the anti-activator QslA negatively regulated PCA biosynthesis and
phz1
expression in strain PA1201 but had little effect on
phz2
expression. This downregulation was mediated by a 56-bp region within the 5'-untranslated region (5'-UTR) of the
phz1
promoter and was independent of
LasR
and RsaL signaling. QslA also negatively regulated
Pseudomonas
quinolone signal (PQS) production. Indeed, QslA controlled the PQS threshold concentration needed for PQS-dependent PCA biosynthesis. The quorum sensing regulator MvfR was required for the QslA-dependent inhibition of PCA production. We identified a direct protein-protein interaction between QslA and MvfR. The ligand-binding domain of MvfR (residues 123-306) was involved in this interaction. Our results suggested that MvfR bound directly to the promoter of the
phz1
cluster. QslA interaction with MvfR prevented the binding of MvfR to the
phz1
promoter regions. Thus, this study depicted a new mechanism by which QslA controls PCA and PQS biosynthesis in
P
.
aeruginosa
.
...
PMID:The Anti-activator QslA Negatively Regulates Phenazine-1-Carboxylic Acid Biosynthesis by Interacting With the Quorum Sensing Regulator MvfR in the Rhizobacterium
Pseudomonas aeruginosa
Strain PA1201. 3009 88
