Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.3.1.177 (BIS)
 957 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The proinflammatory mediator bradykinin (BK) is suggested to play an important role in the pathogenesis of various inflammatory diseases including periodontitis. In this study, BK per se stimulated interleukin-8 (IL-8) production in human gingival fibroblasts in vitro. Furthermore, BK upregulated the stimulatory effect of the cytokines IL-1beta and TNFalpha on the production of IL-8. The stimulatory effect of BK on the IL-1beta- or TNFalpha-stimulated IL-8 production was reduced in the presence of BK B2 receptor antagonist HOE 140, whereas the B1 receptor antagonist Lys-(des-arg9, Leu8)-BK had no effect. Similar to BK, the calcium ionophore A23187 also upregulated the stimulatory effect of IL-1beta and TNFalpha on IL-8 production. The protein kinase C (PKC) inhibitor bisindolylmaleimide, BIS, significantly reduced the stimulatory effect of BK on IL-1beta and TNFalpha increased IL-8 production but did not affect the production of IL-8 stimulated by cytokines alone. The specific p38 mitogen-activated protein kinase (MAPK) inhibitor SB 203580 reduced IL-8 production stimulated by the combination of BK and IL-1beta as well as the IL-1beta-stimulated IL-8 production. In conclusion, this study shows that BK upregulates IL-1beta- and TNFalpha-stimulated IL-8 production via BK B2 receptor and that PKC signal pathway seems to be involved in the upregulation of the cytokine-induced IL-8 production in gingival fibroblasts. This stimulatory effect of BK on IL-8 production may contribute to the maintenance of the gingival inflammation and enhanced risk for destruction of gingival connective tissue.
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PMID:Bradykinin upregulates IL-8 production in human gingival fibroblasts stimulated by interleukin-1beta and tumor necrosis factor alpha. 1566 65

Thermosensitive N-isopropylacrylamide (NIPA)-based hydrogels have been prepared using a biodegradable pseudo-peptide (DMTLT, a tri-molecular adduct of tyrosine, lysine, tyrosine) as cross-linker. This new cross-linker provides a similar cross-linking efficiency as N,N' methylenbisacrylamide (BIS) (a standard biostable cross-linker) used as reference. The amount of DMTLT has shown to modulate, in addition to the cross-linking density, the transition temperature (the higher the amount of DMTLT, the lower the transition temperature), as well as the morphology and the whole aqueous behaviour. The incorporation of hydrophilic N,N'-dimethylacrylamide (DMA) increases the transition temperature, as expected. Finally, the matrices have exhibited in aqueous media a well-defined pulsatile behaviour in swelling and release of benzoic acid and dextran as models of ionisable molecules and non-ionisable macromolecules.
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PMID:Thermosensitivity of N-isopropylacrylamide hydrogels cross-linked with degradable cross-linker. 1853 96