Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.3.1.108 (TAT)
 2,389 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This study investigated whether the immunobead test (IBT) could, for the purposes of simplicity and saving time, be applied directly on an unwashed semen sample instead of washed spermatozoa. These two methods were performed simultaneously on the semen samples of 15 men with a positive MAR test and 10 men with a negative MAR test. A possible association was found between the unwashed samples, showing positive IB binding (greater than 20%) on the tail and/or head and the seminal plasma TAT titers (P less than .00001, Fisher's exact test). In all cases with IB binding of greater than or equal to 20% on unwashed spermatozoa, positive seminal plasma TAT titers (greater than or equal to 32) and SCMC tests (greater than or equal to 50%) were found. In all cases where the binding of the beads was mainly located on the tailtips of the washed or unwashed spermatozoa, negative seminal plasma TAT titers and SCMC tests were found. Coating of the head and/or upper tail regions in both methods was always associated with high TAT titers and a strong-positive SCMC test. It is concluded that the IBT for IgA, but not for IgG, can be performed directly on unwashed semen and that the position of IB binding on the spermatozoa is of prognostic importance with regard to the expected outcome of the SCMC test and seminal plasma TAT titers.
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PMID:Detection of sperm antibodies on unwashed spermatozoa with the immunobead test: a comparison of results with the routine method and seminal plasma TAT titers and SCMC test. 187 57

Twenty-two immunologically infertile couples (male partners had proven autosperm antibodies-positive mixed antiglobulin reaction test [MAR] and direct immunobead test [d-IBT]) were treated with washed spermatozoa used either in the gamete intrafallopian tube transfer (GIFT) or artificial insemination (AIH) procedures. Sixteen of the 22 couples (72.2%) fell pregnant with an ongoing pregnancy rate of 54.5% (12/22). The pregnant and non-pregnant groups were compared with regards to the sperm antibodies detected on the spermatozoa (MAR, d-IBT and sperm cervical mucus contact [SCMC] test) and in the serum and seminal plasma of the male partners (tray agglutination test [TAT], indirect immunobead test [i-IBT], and enzyme-linked immunosorbent assay [ELISA]). The semen parameters, motility, forward progression, count/ml and normal morphological forms were also compared. Statistical analysis showed no difference between the two groups (pregnant and non-pregnant) with regards to the antibody tests performed on semen, serum and seminal plasma. No difference was also seen between the semen parameters of the two groups. The washing of spermatozoa for the GIFT or AIH procedures may therefore be a successful method of treatment for immunologically infertile couples. The results also indicate no difference in the fertility prognosis for the two groups since antibody levels and semen quality were not different between the pregnant and nonpregnant group.
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PMID:Autosperm antibodies in treated, pregnant and non-pregnant immunologically infertile patients. 262 69