Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.108 (
TAT
)
2,389
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Intracellular delivery is a prerequisite for the efficacy of many pharmaceutical proteins. Herein, vitamin B
6
(pyridoxal-5'-phosphate,
PLP
) functionalized calcium phosphate (CP) is used as the bio-recyclable nanocarrier for delivery of proteins into cells. Proteins could be loaded on/released from
PLP
-CP via formation/hydrolysis of pH sensitive aldimine bridging lysine and surface-displayed
PLP
. The loaded proteins could be delivered into the cytosol of HeLa, HepG2 and L929 cells where the carrier could be metabolized into endogenous metabolites of Ca
2+
, HPO
4
2-
, and vitamin B
6
.
PLP
-CP mediated cell transduction is 10-40 folds more efficient than
TAT
which is a widely used cell penetrating peptide, demonstrating the utility of
PLP
-CP as the traceless platform for high-efficiency delivery of proteins into mammalian cells.
...
PMID:Traceless protein delivery with an efficient recyclable nanocarrier. 3248 60
