Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.108 (
TAT
)
2,389
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Sprouty (SPRY) protein negatively modulates fibroblast growth factor and epidermal growth factor actions. We showed that human
SPRY2
inhibits cell growth and migration in response to serum and several growth factors. Using rat intestinal epithelial (IEC-6) cells, we investigated the involvement of the Rho family of GTPases, RhoA, Rac1, and cdc42 in
SPRY2
-mediated inhibition of cell migration and proliferation. The ability of
TAT
-tagged
SPRY2
to inhibit proliferation and migration of IEC-6 cells transfected with constitutively active mutants of RhoA(G14V), Rac1(G12V), and cdc42 (F28L) was determined. Constitutively active RhoA(G14V), Rac1(G12V), or cdc42(F28L) did not protect cells from the anti-proliferative actions of
TAT
-
SPRY2
. The ability of
TAT
-
hSPRY2
to inhibit migration was not altered by of RhoA(G14V) and cdc42(F28L). However, Rac1(G12V) obliterated the ability of
SPRY2
to inhibit cell autonomous or serum-induced migration. Also, the activation of endogenous Rac1 was attenuated by
TAT
-
SPRY2
. Thus,
SPRY2
mediates its anti-migratory actions by inhibiting Rac1 activation.
...
PMID:Sprouty regulates cell migration by inhibiting the activation of Rac1 GTPase. 1535 7
