Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.108 (
TAT
)
2,389
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
Caenorhabditis elegans
aminophospholipid translocase
TAT
-1 maintains phosphatidylserine (PS) asymmetry in the plasma membrane and regulates endocytic transport. Despite these important functions, the structure-function relationship of this protein is poorly understood. Taking advantage of the
tat-1
mutations identified by the
C. elegans
million mutation project, we investigated the effects of 16 single amino acid substitutions on the two functions of the
TAT
-1 protein. Two substitutions that alter a highly conserved PISL motif in the fourth transmembrane domain and a highly conserved DKTGT phosphorylation motif, respectively, disrupt both functions of
TAT
-1, leading to a vesicular gut defect and ectopic PS exposure on the cell surface, whereas most other substitutions across the
TAT
-1 protein, often predicted to be deleterious by bioinformatics programs, do not affect the functions of
TAT
-1. These results provide
in vivo
evidence for the importance of the PISL and DKTGT motifs in P4-type ATPases and improve our understanding of the structure-function relationship of
TAT
-1. Our study also provides an example of how the
C. elegans
million mutation project helps decipher the structure, functions, and mechanisms of action of important genes.
...
PMID:Structure and function analysis of the
C. elegans
aminophospholipid translocase TAT-1. 3068 97
