Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.107 (
DAT
)
1,471
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have substituted deoxyadenosine or adenosine for hypoxanthine in the standard HAT selection system in an attempt to select for
ADA
-normal (ADA+) cells.
ADA
- human lymphoid line cells could not utilize deoxyadenosine as an alternative to hypoxanthine as a purine source (
DAT
) and failed to grow but were only somewhat inhibited in growth when adenosine was substituted for hypoxanthine (AAT). In contrast, ADA+ cells utilized adenosine or deoxyadenosine as efficiently as hypoxanthine as a purine source. Growth in
DAT
, but not in HAT, of an artificial mixture of one ADA+ human lymphoid cells in 1,000
ADA
- cells resulted in enrichment of ADA+ cells to 25-86% of total cells. When we grew a rat
ADA
- cell line in two variations of the
DAT
system, we detected at least three electrophoretically different ADA+ patterns, one of which corresponded to normal rat
ADA
. These could represent "revertants."
...
PMID:An approach to a selection system for adenosine-deaminase-positive (ADA+) cells and detection of rat ADA+ "revertants". 387 5
