Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.1.1.69 (BMT)
 2,655 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

FCM has become an invaluable technique in a variety of clinical and research applications. Then, FCM is used for quantitative analysis of human erythrocyte antigens using Spectrum III. Antibody sensitizations give rise to erythrocyte agglutination making the FCM analysis of a single cell impossible. However, such agglutination could be prevented by some of erythrocyte fixations with PFA and DMS. To make examinations in an optimal concentration of primary antibodies was important for FCM assay. FCM was used to detect small percentage of D antigen positive cell contaminated in negative samples, subsequently positive cells were finally detected in 0.2%. This assay was utilized on a recipient of M/N mismatched allogeneic BMT, and an accurate proof of engraftment was obtained. Relationship of the numbers of antigen determinants and immunofluorescence intensities might be in linear correlation by log-log transformation. The fluorescence intensities of D antigen were analyzed for different rhesus phenotypes and variants such as Du and -D- cells. Lower fluorescence intensities on Du and higher intensities on -D- cell could be seen comparing with average value of D positive cells. However, no relationship between rhesus phenotypes and anti-D immunofluorescence intensities was observed. These findings suggest that FCM is useful and accurate technique for measuring relative antigen determinants of erythrocyte.
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PMID:[The application of flow cytometry in analysis of erythrocyte antigen determinants]. 169 3

Antidepressant activity of N-methyl-D-aspartate (NMDA) receptor antagonists and negative allosteric modulators (NAMs) has led to increased investigation of their behavioral pharmacology. NMDA antagonists, such as ketamine, impair cognition in multiple species and in multiple cognitive domains. However, studies with NR2B subtype-selective NAMs have reported mixed results in rodents including increased impulsivity, no effect on cognition, impairment or even improvement of some cognitive tasks. To date, the effects of NR2B-selective NAMs on cognitive tests have not been reported in nonhuman primates. The current study evaluated two selective NR2B NAMs, CP101,606 and BMT-108908, along with the nonselective NMDA antagonists, ketamine and AZD6765, in the nonhuman primate Cambridge Neuropsychological Test Automated Battery (CANTAB) list-based delayed match to sample (list-DMS) task. Ketamine and the two NMDA NR2B NAMs produced selective impairments in memory in the list-DMS task. AZD6765 impaired performance in a non-specific manner. In a separate cohort, CP101,606 impaired performance of the nonhuman primate CANTAB visuo-spatial Paired Associates Learning (vsPAL) task with a selective impairment at more difficult conditions. The results of these studies clearly show that systemic administration of a selective NR2B NAM can cause transient cognitive impairment in multiple cognitive domains.
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PMID:Negative Allosteric Modulators Selective for The NR2B Subtype of The NMDA Receptor Impair Cognition in Multiple Domains. 2610 37