Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.1.1.69 (
BMT
)
2,655
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Background:
Aflatoxins (AFs) are secondary metabolites of fungi and are one of the causes of toxin-related pet food recalls. An intralaboratory method was previously developed to quantify aflatoxin B
1
(AFB
1
) and aflatoxin M
1
(
AFM
1
) in animal liver by HPLC with fluorescence detection.
Objective:
The aim of this study was to extensively evaluate the method performance with a single-laboratory blinded method test (BMT-S) and a multilaboratory blinded method test (BMT-M).
Methods:
Blinded tissue samples were prepared by a third-party laboratory and sent out to participating laboratories for both
BMT
-S and
BMT
-M.
Results:
In both tests, participants analyzed blinded samples prepared by an independent laboratory. In the
BMT
-S, accuracy ranged between 111 and 154% for AFB
1
and 113 and 159% for
AFM
1
within the quantitation range of 0.1-0.5 ng/g. The HorRat values for repeatability ranged between 0.1 and 0.3 for AFB
1
and 0.3 and 0.6 for
AFM
1
. In the
BMT
-M, the interlaboratory accuracy ranged between 77 and 81% for AFB
1
and 83 and 85% for
AFM
1
within the quantitation range of 0.2-10 ng/g. The HorRat values for reproducibility ranged between 0.4 and 0.7 for AFB
1
and 0.4 and 0.9 for
AFM
1
. Both recovery and reproducibility were acceptable.
Conclusions:
BMT
-M evaluation demonstrated that the method was suitable for quantitation of aflatoxins B
1
and M
1
in animal liver between laboratories.
Highlights:
The
BMT
-S and
BMT
-M results demonstrated that the method is rugged and reproducible among the participating laboratories.
...
PMID:Evaluation of a Diagnostic Method to Quantify Aflatoxins B
1
and M
1
in Animal Liver by High-Performance Liquid Chromatography with Fluorescence Detection. 3073 68
