Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.1.1.37 (
DNA methyltransferase
)
4,983
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Several enzymes, for example
DNA (cytosine-5-)-methyltransferase
, produce relatively strong interactions with DNA and hinder the quantitative recovery of this DNA from a reaction mixture. Classical methods like the Sevag chloroform-iso-amylic alcohol one or the phenol procedure lead only to a 50-60 per cent recovery of the DNA. A new procedure was worked out utilizing pancreatic RNase,
proteinase K
, NaOH 0.5 M treatment and trichloracetic acid precipitation which gives 85 per cent recovery of DNA.
...
PMID:Comparison of different methods of recovering DNA from a methylation assay. 628 57
