Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.1.1.148 (
Thy1
)
1,210
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The guanine nucleotide exchange factor
C3G
is one of the mediators of endothelin-1 (ET-1) intracellular signaling cascades and is vital for kidney development and homeostasis. The aim of the current study was to analyze the specificity of ET-1-induced signaling via
C3G
in rat glomerular mesangial cells (GMC) and to investigate the biological significance of
C3G
during mesangioproliferative glomerulonephritis. In GMC,
C3G
expression was increased (1) in vivo after induction of the anti-
Thy1
model of glomerulonephritis and (2) in cell culture experiments after fetal bovine serum incubation. To examine the consequences of
C3G
up-regulation, adenovirus-mediated gene transfer of
C3G
into cultured glomerular cells was done, and the GTP loading of the small G proteins Rap1 and R-Ras was analyzed. Overexpression of
C3G
in mesangial cells resulted in enhanced activation of Rap1, but failed to affect the GTP-bound status of R-Ras in ET-1-stimulated cells.
C3G
overexpression led to significant changes in GMC spreading and migration patterns in response to ET-1 stimulation and increased stress fiber formation, which was mimicked by Rap1A overexpression. Together, these findings suggest (1) the existence of regulatory mechanisms resulting in disease-related up-regulation of
C3G
in GMC and (2) that an increase in the C3G protein level may contribute to the resolution stage of mesangioproliferative glomerulonephritis by reducing GMC sensitivity to ET-1, modulating cellular motility, and actin dynamics.
...
PMID:Endothelin signaling via guanine exchange factor C3G in renal glomerular mesangial cells. 2072 39
