Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.1.1.113 (restriction-modification system)
 350 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The AgeI restriction-modification system from a marine bacterium, Agrobacterium gelatinovorum IAM 12617, recognizes the nucleotide sequence ACCGGT. The gene coding for the AgeI methylase (M.AgeI) was cloned into Escherichia coli DH5 alpha MCR, and the nucleotides of the gene were sequenced. The M.AgeI gene coded for a protein of 429 amino acid residues (molecular mass, 47,358 daltons). The deduced amino acid sequence of M.AgeI was compared with those of other methylases and showed that there are high degrees of similarity in some cytosine-5 methylases.
 ...
PMID:Cloning and nucleotide sequence of the AgeI methylase gene from Agrobacterium gelatinovorum IAM 12617, a marine bacterium. 890 Nov 2

The ApaLI restriction-modification system from Acetobacter pasteurianus IFO 13753 recognizes the nucleotide sequence GTGCAC. The gene coding for the ApaLI methylase (M.ApaLI) was cloned into Escherichia coli DH5 alpha MCR, and the nucleotide sequence of the gene was analyzed. The M.ApaLI gene coded for a protein of 429 amino acid residues (molecular mass, 46,554 daltons). The ApaLI restriction endonuclease (R.ApaLI) gene was analyzed by inverse polymerase chain reaction. The R.ApaLI gene coded for a protein of 375 amino acid residues (molecular mass, 42,143 daltons). The two genes had the same orientation separated by two base pairs. The deduced amino acid sequence of M.ApaLI shows significant similarities to the family of cytosine-5 methylases. However, the deduced amino acid sequence of R.ApaLI did not have as much relatedness in the nucleotide sequence, when compared with those of the other restriction endonucleases already reported.
 ...
PMID:Cloning and nucleotide sequence of ApaLI restriction-modification system from Acetobacter pasteurianus IFO 13753. 898 85