Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.8.1.4 (diaphorase)
 2,754 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This study has examined the development of cells in the rat retina which contain nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase. NADPH-diaphorase cells were first detected at postnatal day (P) 3, in somata located in the inner part of the cytoblast layer (CBL). At this age, NADPH-diaphorase reactivity was also seen in weakly labelled fibers in the presumptive outer plexiform layer (OPL). By P5, the somata of most labelled cells were in the inner part of the inner nuclear layer (INL), and by P11, their processes had spread extensively within the inner plexiform layer (IPL). By P25, there was a striking change in the pattern of NADPH-diaphorase reactivity. First, cells had lost reactivity from their large and extensive dendrites and second, there was a distinct reduction in the diameters of labelled somata. Thus, NADPH-diaphorase reactivity was most prominent during the period of synaptogenesis in the IPL. Labelled cells at P3 numbered 120 and were largely found at the superior margin of the retina. By P11, their total number had increased to the adult value of about 3400 and their density was highest in peripheral retina. With further development, the differential expansion of the retina appeared to lower the peripheral densities, resulting in an approximately uniform distribution by adulthood.
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PMID:Development of NADPH-diaphorase cells in the rat's retina. 281 96

Since nitric oxide has a role in the refinement of the retinal projection to the superior colliculus (SC), we studied the onset of neuronal nitric oxide synthase (nNOS) expression in the mouse SC in order to compare its development with that of the refinement process. Sections from animals at ages P1, P5, P8, P11, P15, and P21 and adults were examined with nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) histochemistry or immunocytochemistry using an antibody directed against nNOS. At all ages there was a wedge of labeled neurons in the dorsolateral periaqueductal gray extending into the deep layers of the SC. At P1 there was also a single superficial band of labeled neurons within the region that will become the intermediate gray layer (IGL). By P5, labeled neurons were also seen in what will become the superficial gray layer. There was a ventral to dorsal progression in nNOS expression with substantial changes in the numbers of labeled neurons in the different laminae between P5 and adulthood. The number of labeled neurons in the IGL peaked at P15, whereas in the superficial layers the numbers continued to increase through P21 and then declined in adults. At all ages these neurons represented a variety of morphological cell types. The onset of nNOS expression in the different laminae is earlier than has been reported in studies using NADPHd as a marker for nNOS. The temporal and spatial patterns of nNOS expression reported here match more closely the time course of pathway refinement in the SC, providing additional evidence for the involvement of nitric oxide in this process.
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PMID:Postnatal development of nitric oxide synthase expression in the mouse superior colliculus. 1105 65