Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
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Target Concepts:
Gene/Protein
Disease
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Query: EC:1.7.1.4 (
nitrite reductase
)
1,847
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Hydrogenobacter thermophilus strain TK-6 was observed to grow anaerobically on nitrate as an electron acceptor when molecular hydrogen was used as an energy source. Nitrite was detected as the product of a respiratory reaction. 15NO, 15N2O, and 15N2 were detected with Na15NO3 as an electron acceptor. Western immunoblot analysis showed that cell-free extracts from cells grown on nitrate reacted with antibodies against heme cd1-type
nitrite reductase
from Pseudomonas aeruginosa. The positive bands, which had molecular masses similar to that of the heme cd1-type
nitrite reductase
, were also stained by heme staining. These results indicate that
nitrite reductase
of strain TK-6 is a heme cd1-type enzyme. Activity of
ATP:citrate lyase
, one of the key enzymes of the reductive TCA cycle, was detected in cell-free extract of cells cultivated on nitrate, which indicates that the cycle operates during anaerobic growth.
...
PMID:Nitrate respiratory metabolism in an obligately autotrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6. 1127 24
