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Query: EC:1.7.1.4 (nitrite reductase)
 1,847 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Using tobacco as a model species, we have developed a simple procedure for the selection of spontaneous haploid plants under horticultural conditions, which does not require the use of any selective agent. One transgenic tobacco plant, homozygous for an antisense transgene able to silence the expression of nitrite reductase host genes, and encoding the second enzyme of the nitrate assimilation pathway, was used to pollinate two different cultivars of wild type tobacco plants. Seeds were sown at high density in the greenhouse and watered with a nutrient solution containing nitrate. Green plants able to develop normally emerged at a frequency of 5.10(-4) in a mass of chlorotic retarded plants. Phenotypic and genetic analysis, chloroplast counting in stomatal guard cells and molecular hybridizations revealed that 22% of these plants were gynogenetic haploid plants exhibiting the maternal phenotype whereas the remaining 78% were true diploid plants that have lost the antisense transgene. These results demonstrate that a transgene able to silence the expression of a housekeeping gene can be utilized as a counter-selectionable marker for the rapid and easy selection of spontaneous haploid plants in transformable species.
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PMID:Nitrite reductase silencing as a tool for selecting spontaneous haploid plants. 2418 45

In the context of studying the bacterial community involved in nitrogen transformation processes in arable soils exposed to different extents of erosion and sedimentation in a long-term experiment (CarboZALF), a strain was isolated that reduced nitrate to nitrous oxide without formation of molecular nitrogen. The presence of the functional gene nirK, encoding the respiratory copper-containing nitrite reductase, and the absence of the nitrous oxide reductase gene nosZ indicated a truncated denitrification pathway and that this bacterium may contribute significantly to the formation of the important greenhouse gas N2O. Phylogenetic analysis based on the 16S rRNA gene sequence and the housekeeping genes recA and atpD demonstrated that the investigated soil isolate belongs to the genus Rhizobium. The closest phylogenetic neighbours were the type strains of Rhizobium. subbaraonis and Rhizobium. halophytocola. The close relationship with R. subbaraonis was reflected by similarity analysis of the recA and atpD genes and their amino acid positions. DNA-DNA hybridization studies revealed genetic differences at the species level, which were substantiated by analysis of the whole-cell fatty acid profile and several distinct physiological characteristics. Based on these results, it was concluded that the soil isolate represents a novel species of the genus Rhizobium, for which the name Rhizobium azooxidifex sp. nov. (type strain Po 20/26T=DSM 100211T=LMG 28788T) is proposed.
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PMID:Characterization of the N2O-producing soil bacterium Rhizobium azooxidifex sp. nov. 2703 Sep 72