Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: EC:1.7.1.4 (
nitrite reductase
)
1,847
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The selective and efficient nitrite reduction process is ubiquitous in biological systems. To understand copper-mediated nitrite reduction, we developed a bio-inspired model system to investigate the mechanism of copper-containing
nitrite reductase
. A well-characterized copper(i)-nitrate complex with amino functionalized 2-(diphenylphosphino)
aniline
ligands, [(Ph
2
PC
6
H
4
(o-NH
2
))
2
Cu(ONO)], demonstrated the
aniline
protonation will cause NO release in an acidic environment. To further understand NO releasing ability, we also performed pH-dependency experiments and confocal imaging to release NO under physiological buffer conditions. According to titration and spectroscopic studies on the protonation reaction of complex [(Ph
2
PC
6
H
4
(o-NH
2
))
2
Cu(ONO)], we proposed a mechanistic pathway for proton transfer and NO release. Furthermore, DFT calculations predicted that the release of NO takes place via
aniline
in both organic and aqueous media. These results highlight the importance of the proton-rich microenvironment around the copper(i)-nitrite core to induce nitrate reduction in a chemical and biological environment.
...
PMID:Nitric oxide-release study of a bio-inspired copper(i)-nitrito complex under chemical and biological conditions. 3017 63
