Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.7.1.2 (
nitrate reductase
)
3,861
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Barley (Hordeum vulgare L.) has both NADH-specific and NAD(P)H-bispecific nitrate reductases. Genomic and cDNA clones of the NADH
nitrate reductase
have been sequenced. In this study, a
genomic clone
(pMJ4.1) of a second type of
nitrate reductase
was isolated from barley by homology to a partial-length NADH
nitrate reductase
cDNA and the sequence determined. The open reading frame encodes a polypeptide of 891 amino acids and its interrupted by two small introns. The deduced amino acid sequence has 70% identity to the barley NADH-specific
nitrate reductase
. The non-coding regions of the pMJ4.1 gene have low homology (ca. 40%) to the corresponding regions of the NADH
nitrate reductase
gene. Expression of the pMJ4.1
nitrate reductase
gene is induced by nitrate in root tissues which corresponds to the induction of NAD(P)H
nitrate reductase
activity. The pMJ4.1
nitrate reductase
gene is sufficiently different from all previously reported higher plant
nitrate reductase
genes to suggest that it encodes the barley NAD(P)H-bispecific
nitrate reductase
.
...
PMID:Characterization and sequence of a novel nitrate reductase from barley. 189 7
In this paper, we describe the gene (nia) coding for the apoenzyme of the
nitrate reductase
(NR) of petunia. A full-size
genomic clone
was isolated from a genomic library, using the tobacco nia2 cDNA as a probe, and sequenced. The open reading frame is interrupted by three introns and encodes a protein of 909 amino acids which reveals between 92% and 68% identity to the NADH NR apoenzyme from other higher plants. Southern analyses indicated that the NR apoenzyme is encoded by a single-copy gene, although another region homologous to part of nia was also identified. The analysis of the steady-state level of nia mRNA showed that the petunia nia is regulated by the nitrogen source and is under the control of the circadian rhythm.
...
PMID:Analysis of the petunia nitrate reductase apoenzyme-encoding gene: a first step for sequence modification analysis. 851 83
