Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.7.1.2 (
nitrate reductase
)
3,861
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Centipedegrass (
Eremochloa ophiuroides
[Munro] Hack.) is an important warm-season turfgrass species. Transgenic centipedgrass plants overexpressing
S-adenosylmethionine decarboxylase
from bermudagrass (
CdSAMDC1
) that was induced in response to cold were generated in this study. Higher levels of
CdSAMDC1
transcript and sperimidine (Spd) and spermin (Spm) concentrations and enhanced freezing and chilling tolerance were observed in transgenic plants as compared with the wild type (WT). Transgenic plants had higher levels of polyamine oxidase (PAO) activity and H
2
O
2
than WT, which were blocked by pretreatment with methylglyoxal bis (guanylhydrazone) or
MGBG
, inhibitor of SAMDC, indicating that the increased PAO and H
2
O
2
were a result of expression of
CdSAMDC1
. In addition, transgenic plants had higher levels of
nitrate reductase
(NR) activity and nitric oxide (NO) concentration. The increased NR activity were blocked by pretreatment with
MGBG
and ascorbic acid (AsA), scavenger of H
2
O
2
, while the increased NO level was blocked by
MGBG
, AsA, and inhibitors of NR, indicating that the enhanced NR-derived NO was dependent upon H
2
O
2
, as a result of expression
CdSAMDC1
. Elevated superoxide dismutase (SOD) and catalase (CAT) activities were observed in transgenic plants than in WT, which were blocked by pretreatment with
MGBG
, AsA, inhibitors of NR and scavenger of NO, indicating that the increased activities of SOD and CAT depends on expression of
CdSAMDC1
, H
2
O
2
, and NR-derived NO. Our results suggest that the elevated cold tolerance was associated with PAO catalyzed production of H
2
O
2
, which in turn led to NR-derived NO production and induced antioxidant enzyme activities in transgenic plants.
...
PMID:Transgenic Centipedegrass (
Eremochloa ophiuroides
[Munro] Hack.) Overexpressing
S
-Adenosylmethionine Decarboxylase (SAMDC) Gene for Improved Cold Tolerance Through Involvement of H
2
O
2
and NO Signaling. 2901 65
