Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.7.1.2 (nitrate reductase)
 3,861 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The study of 52 strains of rapidly growing mycobacteria showed that Mycobacterium fortuitum and M. chelonei were clearly distinguished by the aid of seven key tests (nitrate reductase, iron uptake, beta-glucosidase, penicillinase, growth on fructose, resistance to pipemidic acid, and resistance to capreomycin) and by analysis of their respective mycolic acids. However, the subdivision of these species into M. fortuitum var. fortuitum and M. fortuitum var. peregrinum and M. chelonei subsp. chelonei and M. chelonei subsp. abscessus was not satisfactorily accomplished.
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PMID:Identification of Mycobacterium fortuitum and Mycobacterium chelonei. 619 Aug 37

Mycobacterium fortuitum and Mycobacterium chelonei are distinguished unambiguously by the combined use of five test characters: nitrate reductase, beta-glucosidase, acid production from fructose, penicillinase, and trehalase. Typically, M. fortuitum was nitrate reductase positive, beta-glucosidase positive; M. chelonei was nitrate reductase negative, beta-glucosidase negative, penicillinase positive, and trehalase positive and did not produce acid from fructose.
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PMID:Differential identification of Mycobacterium fortuitum and Mycobacterium chelonei. 678 Jun 4

Whole cells of Mycobacterium avium, characterized by their negative response in the nine biochemical tests used for mycobacterial identification in our laboratory, turned positive for nitrate reductase, Tween-80 hydrolysis, beta-glucosidase, acid phosphatase, alkaline phosphatase, penicillinase, and trehalase after their wall portion was removed to yield spheroplasts. This suggested that the negative results in most of the biochemical procedures were caused by the exclusion mechanism at the wall level. Preliminary transmission and scanning electron microscopic studies showed differences at wall level between laboratory-maintained opaque, dome-shaped (SmD) and host-recycled smooth, transparent (SmT) colony type variants of M. avium and suggested the presence of an outer regularly structured layer in SmT variants. Comparative ultrastructural studies utilizing different polysaccharide coloration methods confirmed the presence of an outer polysaccharide layer in SmT variants which was probably related to their enhanced pathogenicity for experimental animals and drug resistance as compared to that of SmD variants. These findings are discussed with respect to multiple drug resistance, virulence, and gene expression of M. avium.
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PMID:Multiple drug resistance in Mycobacterium avium: is the wall architecture responsible for exclusion of antimicrobial agents? 679 25

Biotyping of Haemophilus influenzae into five type and H. parainfluenzae into three types based on indole production, ornithine decarboxylase, and urease has been reported (M. Kilian, Acta Pathol. Microbiol. Scand. Sect. B 82:835--842, 1976). A commercially available test system designed for the 4-h identification of Enterobacteriaceae. Micro-ID, proved efficacious for the rapid biotyping of these two Haemophilus species. The nitrate reductase, indole production, ornithine decarboxylase, urease, and o-nitrophenyl-beta-D-galactopyranoside hydrolysis tests in Micro-ID correlated over 99% with conventional methodology. By utilizing the indole and o-nitrophenyl-beta-D-galactopyranoside tests it was possible, with 261 of 272 (96.1%) isolates, to distinguish H. influenzae from H. parainfluenzae. Cerebrospinal fluid isolates were over 90% H. influenzae biotype I, and conjunctival isolates were approximately 70% biotype II. Type b H. influenzae were predominantly biotypes I and II; these type b isolates were also overwhelmingly indole producers. Although over 90% of biotypes I and II have been reported to produce beta-lactamase, this was not confirmed by the small number of beta-lactamase producers encountered here. The 4-h Micro-ID should prove a useful mechanism, amenable to the routine clinical laboratory, for the further exploration of the association of Haemophilus with the site of isolation, antigenicity, and antibiotic resistance.
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PMID:Rapid biochemical characterization of Haemophilus species by using the micro-ID. 698 1

We have isolated two phenotypically distinct nonfastidious Francisella strains (Fx1 and Fx2) from the blood of compromised patients with pneumonia and compared them with eight other Francisella strains, including Francisella tularensis biovar tularensis, F. tularensis biovar novicida, and F. philomiragia. Our isolates grew well on sheep blood agar, chocolate agar, modified Thayer-Martin agar, and Trypticase soy agar. Fx1 and Fx2 were determined to be within the Francisella genus by cellular fatty acid analysis and by the utilization of glucose, production of H2S and catalase, and lack of motility, oxidase, nitrate reductase, and gelatinase. They were additionally shown to belong to the species F. tularensis by sequencing of two variable regions comprising approximately 500 nucleotides of the 16S rRNA gene. Also, RNA probe hybridization confirmed their belonging to the species F. tularensis. However, the new strains, which are not identical, are distinguished from other F. tularensis strains by growth characteristics, repetitive extragenic palindromic PCR fragment pattern, and some biochemical tests. Key biochemical differences included the findings that Fx1 was positive for beta-galactosidase and arabinose hydrolysis and that both strains were citrulline ureidase positive and glycerol negative. Commercial F. tularensis antiserum agglutinated stock F. tularensis strains but not Fx1, Fx2, F. tularensis biovar novicida, or F. philomiragia; serum from either patient failed to agglutinate or only weakly agglutinated commercial antigen but showed agglutination when tested against each patient's respective isolate. Fx1 and Fx2 produced beta-lactamase. Because of their good growth, negative serology, and biochemical profile, the organisms could be misidentified in the clinical laboratory if standard strategies or commercial identification systems are used.
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PMID:Characterization of two unusual clinically significant Francisella strains. 881 97