Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.7.1.2 (
nitrate reductase
)
3,861
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The immunoaffinity chromatography of total membrane proteins from Escherichia coli helped determine the specificity of the monoclonal antibody 3A6 that was obtained upon immunization of mice with
nicotinamide nucleotide transhydrogenase
preparations and reacted with an unknown E. coli antigen. Proteins with apparent molecular masses of 150, 45, and 20 kDa were isolated and identified by N-terminal sequencing as the subunits of
nitrate reductase
. This conclusion was confirmed by immunoblotting with the 3A6 antibody of the proteins from the E. coli cells grown upon induction of
nitrate reductase
. It was shown that the 3A6 antibody specifically recognizes the alpha subunit of
nitrate reductase
, and the formation of the enzyme-antibody complex does not result in a loss of the enzyme catalytic activity.
...
PMID:[Identification of Escherichia coli nitrate reductase as an antigen for a monoclonal antibody with previously unknown specificity]. 1104 Sep 97
