Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.7.1.1 (
nitrate reductase
)
3,728
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The microbial ecology of "soppressata of Vallo di
Diano
," a traditional dry fermented sausage from southern Italy, was studied by using both culture-dependent and culture-independent approaches. The ripened fermented sausages were characterized by high microbial loads of both staphylococci and lactobacilli. Using PCR-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the variable V3 and V1 regions of the 16S rRNA gene and direct DNA sequencing, it was possible to identify Staphylococcus xylosus, S. succinus, and S. equorum among the staphylococci and Lactobacillus sakei and L. curvatus within the lactobacilli. Moreover, Debaryomyces hansenii was the main yeast species found by targeting the yeast 26S rRNA gene by PCR-DGGE. Selected strains of S. xylosus, L. sakei, and L. curvatus were characterized for their technological properties in the ripening conditions of the fermented sausages so as to select an autochthonous starter formulation. The selection included the determination of
nitrate reductase
, lipolytic, and antioxidant activity and proteolysis with myofibrillar and sarcoplasmic protein fractions. Such properties were evaluated in both in vitro and in situ assays; the latter were performed by using each strain as a starter in the laboratory-scale manufacture of soppressata of Vallo di
Diano
and by monitoring the microbiological and chemical changes at the end of ripening. The results show differences between the in vitro and in situ selection results and indicate that in situ evaluation of the technological performance of specific strains is better suited to selecting autochthonous starter cultures for fermented-meat products than in vitro evaluation.
...
PMID:Microbial ecology of the soppressata of Vallo di Diano, a traditional dry fermented sausage from southern Italy, and in vitro and in situ selection of autochthonous starter cultures. 1761 25
Bisphenol A
(
BPA
) is an environmental endocrine disruptor that seriously threatens ecological systems. Plants are the primary producers in ecological systems, but little information is available concerning the toxic effect of
BPA
on plants. In the present study, the effects of
BPA
on the growth and nitrogen nutrition of roots of soybean seedlings were investigated by using a root automatic scan apparatus and biochemical methods. It was found that when soybean seedlings were treated with 1.5 mg/L
BPA
, the growth of roots was improved, the content of nitrate in roots was increased, the content of ammonium in roots was decreased, and the activities of
nitrate reductase
and nitrite reductase in roots were not changed. The opposite effects were observed in roots treated with 17.2 mg/L and 50.0 mg/L
BPA
, except for an increase in the content of nitrate in roots treated with 17.2 mg/L
BPA
and a decrease in the activities of
nitrate reductase
and nitrite reductase in roots of soybeans seedlings. Statistical analysis indicated that the change in the nitrogen nutrition of roots of soybean seedlings treated with
BPA
was one reason why the growth of roots was changed. The authors suggest that the potential environmental and ecological risk of
BPA
to plants should receive more consideration.
...
PMID:Effects of bisphenol A on growth and nitrogen nutrition of roots of soybean seedlings. 2310 93
