Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.7.1.1 (
nitrate reductase
)
3,728
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Pharmacological data have suggested the involvement of mitogen-activated protein kinase (MPK) cascades in dark-induced stomatal closure, but which specific MPK cascade participates in the darkness guard cell signaling and its relationship with hydrogen peroxide (H
2
O
2
) and nitric oxide (NO) remain unclear. In this paper, we observed that darkness induced activation of MPK6 in leaves of wild-type Arabidopsis (Arabidopsis thaliana) and mutants for
nitrate reductase
1 (NIA1), but this effect was inhibited in mutants for MPK Kinase 1 (
MEK1
) and ATRBOHD/F. Mutants for
MEK1
, MPK6 and NIA1 showed defect of dark-induced NO production in guard cells and stomatal closure, but were normal in the dark-induced H
2
O
2
generation, while stomata of mutant AtrbohD/F showed defect of dark-induced H
2
O
2
and NO production and subsequent closure. Moreover, exogenous NO rescued the defect of dark-induced stomatal closure in mutants of AtrbohD/F, mek1 and mpk6, while exogenous H
2
O
2
could not rescue the defect of dark-induced stomatal closure in mutants of mek1, mpk6 and nia1. These genetic and biochemical evidences not only show that
MEK1
-MPK6 cascade, AtRBOHD/F-dependent H
2
O
2
and NIA1-dependent NO are all involved in dark-induced stomatal closure in Arabidopsis, also indicate that
MEK1
-MPK6 cascade functions via working downstream of H
2
O
2
and upstream of NO.
...
PMID:Role and interrelationship of MEK1-MPK6 cascade, hydrogen peroxide and nitric oxide in darkness-induced stomatal closure. 2871 16
