Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.6.99.5 (NADH dehydrogenase)
 2,135 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Genome scans have been used in the studies of ecological speciation to find genomic regions ('outlier loci') showing reduced gene flow between divergent populations/species. High-throughput sequencing ('454') offers new opportunities in this field via transcriptome sequencing. Divergent ecotypes of the marine gastropod Littorina saxatilis represent a good example of incipient ecological speciation. We performed a 454-based genome scan between H and M ecotypes of L. saxatilis from the British Isles using cDNA of pooled individuals. Allele frequencies were calculated for 2454 single nucleotide polymorphisms (SNPs), within 572 contigs, and 7% of loci were detected as outliers. Functional annotation of the contigs containing outlier SNPs showed that they included shell matrix and muscle proteins (lithostathine, mucin, titin), proteins involved in energetic metabolism (arginine kinase, NADH dehydrogenase) and reverse transcriptases. Follow-up investigations into these proteins and unannotated outliers will be a promising route in the study of ecological speciation in L. saxatilis.
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PMID:An EST-based genome scan using 454 sequencing in the marine snail Littorina saxatilis. 2069 60

For centuries, edible bird's nest (EBN) has been consumed as a Chinese delicacy. In the past decades, numerous studies reported that water soluble extract of the EBN not only possessed epidermal growth factor, but also associated with a wide range of health-promoting effects. However, based on the traditional Chinese way of EBN preparation and consumption, the bioactive components should be originated from both its hot water soluble and insoluble fractions. Nevertheless, information on the hot water insoluble fraction (HWIF) of EBN is not currently available. In this study, peptides released from the HWIF of EBN under simulated gastro-intestinal conditions were identified for the first time by de novo sequencing using a combination of MALDI TOF/TOF MS and ESI-ion-trap MS/MS. The released peptides were found to share very high similarities to mucin, NADH dehydrogenase, acidic mammalian chitinase-like protein, immunoglobulin, proline-rich protein, von Willebrand factor and epidermal growth factor domain-containing protein.
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PMID:Identification of peptides released from hot water insoluble fraction of edible bird's nest under simulated gastro-intestinal conditions. 2954 34