Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.6.99.3 (diaphorase)
 5,903 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A study of the ultrastructural localization of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), glucose-6-phosphate dehydrogenase (G-6-PD), beta-hydroxybutyrate dehydrogenase (beta-HBD), NADH diaphorase (NADH-D) and NADPH diaphorase (NADPH-D) in the guinea-pig testis is reported. The procedures employed included short immersion or perfusion fixation with aldehydes followed by incubation of small blocks in a tetrazolium salt or a ferricyanide medium. The effects of incubation conditions were investigated, and a reaction medium for the ultracytochemical demonstration of 11 beta-HSD is described. Using suitable controls, evidence for the specificity of the cytochemical reactions is presented. It was found that all the enzymes studied were present in both the Leydig and Sertoli cells of the guinea-pig testis and that the intracellular distribution pattern for each enzyme was independent of the cell type. Using tetrazolium salt techniques, both 3 beta-HSD and 11 beta-HSD activities were localized on or in membranes of smooth endoplasmic reticulum and within the mitochondria. With the ferricyanide techniques, G-6-PD activity was found to be associated mainly with the smooth endoplasmic reticulum membranes, while beta-HBD activity was limited to mitochondria. With both the tetrazolium salt and ferricyanide techniques, the reaction products for NADH-D and NADPH-D activities showed localizations which were similar to those observed for the steroid dehydrogenases.
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PMID:The ultrastructural localization of the enzymes related to steroid hormone metabolism in the guinea-pig testis. 42 99

The ovary of the domestic pigeon, Columba livia, has been assayed histochemically for the localization of delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-HSDH), 17 beta-hydroxysteroid dehydrogenase (17 beta-HSDA), 11 beta-hydroxysteroid dehydrogenase (11 beta-HSDH), glucose-6-phosphate dehydrogenase (G6P-DH) and NADH-diaphorase activities during different periods of the reproductive cycle. delta 5-3 beta-HSDH, 17 beta-HSDH, 11 beta-HSDH, G6P-DH and NADH-diaphorase activity was found in the theca interna of growing, atretic and postovulatory follicles, the granulosa of ovulatory, atretic and postovulatory follicles, and interstitial gland cells during the pre-incubation and the laying periods. During the incubation and squab feeding periods only delta 5-3 beta-HSDH, G6P-DH and NADH-diaphorase activities were observed in the above mentioned cells. The steroidogenic potential of atretic follicles depends upon the type of atresia a follicle undergoes.
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PMID:Steroid synthesizing cellular sites in the ovary of the domestic pigeon Columba livia (Gmelin): a histochemical study. 45 38

A histochemical study of steroid synthesizing cellular sites in the ovaries of Calotes versicolor (Daud.), Hemidactylus flavivirdes (Ruppel) and Chamaeleon calcaratus (Boulenger) is discussed. THe distribution of delta 5-3beta-hydroxysteroid dehydrogenase, 17beta-hydroxysteroid dehydrogenase, 11beta-hydroxysteroid dehydrogenase, glucose-6phosphate dehydrogenase, isocitrate dehydrogenase, lactate dehydrogenase and reduced nicotinamide-adenine dinucleotide diaphorase enzyme activities was studied in ovaries of the 3 species of lizards. All the enzyme activities occurred in 1) patches of cells of theca interna; 2) granulosa cells of large preovulatory, postovulatory, and atretic follicles; 3) interstitial cells of the ovarian stroma; and in the 4) ooplasm of the growing oocyte, suggesting their steroidogenic capacity. It was observed that following completion of follicular atresia, the phagocytic granulosa cells degenerate and the remaining cells of theca interna contribute to the formation of interstitial gland cells.
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PMID:Steroid synthesizing cellular sites in the ovaries of Calotes versicolor (Daud.), Hemidactylus flaviviridis (Ruppel) & Chamaeleon calcaratus (Boulenger): histochemical study. 102 92