Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.6.99.3 (diaphorase)
 5,903 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Nerve cell bodies projecting to the ovary were visualized in dorsal root ganglia (DRG) and paravertebral ganglia after application of the retrograde tracer Fluoro-gold to the superior ovarian and plexus nerves. The location of fluorescent cells in sections of ganglia was recorded and subsequently nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry was utilized to locate nitric oxide synthase (NOS) whose presence indicates sites where nitric oxide (NO) can be synthesized. No fluorescent nerve cell bodies in paravertebral ganglia were NADPH-diaphorase-positive. In contrast, numerous Fluoro-gold-labeled nerve cell bodies in DRG at segmental levels T12-L1 were NADPH-diaphorase positive. Thus, many sensory neurons projecting to the ovary contain NOS and presumably release NO. This gaseous messenger molecule may participate in modulation of ovarian function.
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PMID:NADPH-diaphorase-positive neurons innervating the rat ovary. 752 6

The histological detection of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d), a marker for nitric oxide-producing cells, was used to evaluate ongoing changes in the neural biochemistry of the rat spinal cord 1 week following contusive spinal cord injury (SCI). In addition, the immunohistochemical detection of the immediate-early gene c-fos was used to identify basal patterns of neural activity at this time. The numbers and laminar locations of NADPH-d- and c-fos-positive cells were examined in spinal segments adjacent to the site of injury (T12-S3) as well as those distant from the injury (C3-C5) in both SCI and un-injured rats. Our data show that contusive SCI results in a significant reduction in NADPH-d labelling in the superficial dorsal horn, and a significant increase in NADPH-d expression in small bipolar neurons and large motoneurons in the ventral horn at the site of the injury. In spinal segments distant to the injury site (C3-C5), NADPH-d activity did not differ from that of uninjured controls. Furthermore, significant reductions in the levels of c-fos expression were observed in SCI rats, in spinal segments both at and distant to the site of injury for all spinal laminae. The only exception was a dramatic increase observed in the sacral parasympathetic nucleus. These data suggest that increased NADPH-d expression is related to conditions specific to the site of injury, whereas the changes in c-fos expression probably indicate more global changes in neuronal activity following SCI.
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PMID:Contusive spinal cord injury evokes localized changes in NADPH-d activity but extensive changes in Fos-like immunoreactivity in the rat. 1758 82