Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.6.99.3 (diaphorase)
 5,903 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Microvillar vesicles have been isolated from rat placentae. The vesicles have been characterized with respect to their microscopic appearance, their capacity to accumulate amino acids, and their enzyme activities. Alkaline phosphatase activity in the vesicle fraction was threefold greater than in the homogenate, and cytochrome c reductase activity was decreased fourfold. The vesicles were used to characterize transferrin binding to the trophoblast membrane in vitro. Transferrin binding was specific (K alpha = 8 X 10(7) M-1), and could not be replaced by apotransferrin. Apotransferrin binding showed lower affinity than the diferric transferrin, and did not appear to saturate over the range 0 to 500 micrograms/ml.
Placenta
PMID:Transferrin binding by microvillar vesicles isolated from rat placenta. 609 Oct 92

Mitochondrial respiratory chain enzyme Complexes are present in placenta at proportion similar to other tissues with exception of glycerophosphate dehydrogenase (mGPDH) which is expressed at a very high rate. As shown by Western blot quantification and respiratory chain enzyme activity measurements, the specific content of mGPDH is similar to that of succinate dehydrogenase or NADH dehydrogenase. Using fluorometric probe dichlorodihydrofluorescein diacetate we found that placental mitochondria display high rate of glycerophosphate-dependent hydrogen peroxide production. This was confirmed by oxygraphic detection of glycerophosphate-induced, KCN- or antimycin A-insensitive oxygen uptake. Hydrogen peroxide production by mGPDH was highly activated by one-electron acceptor, potassium ferricyanide and it was depressed by inhibitors of mGPDH and by cytochrome c. Our results indicate that mGPDH should be considered as an additional source of reactive oxygen species participating in induction of oxidative stress in placenta.
Placenta
PMID:Specific properties of heavy fraction of mitochondria from human-term placenta - glycerophosphate-dependent hydrogen peroxide production. 1594 44