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Target Concepts:
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Query: EC:1.6.99.1 (
NADPH-diaphorase
)
3,903
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
NADPH diaphorase
activity in the rabbit retina is modulated by the state of visual adaptation. In this study, we tested possible glutamatergic control of this phenomenon. Rabbits were injected intravitreally with agonists and antagonists of glutamate. After adaptation (3 hours) to either room light or darkness, the rabbits were killed and the retinae were prepared for
NADPH diaphorase
histochemistry. Kainic acid significantly reduced the number of
NADPH diaphorase
amacrine cells but augmented
NADPH diaphorase
activity in horizontal cells in both light- and dark-adapted animals. 6,7-Dinitroquinoxaline-2,3(1H,
4H)
-dione exerted no effect on amacrine cells but eliminated
NADPH diaphorase
activity in horizontal cells. 2-Amino-4-phosphono butyric acid did not affect
NADPH diaphorase
activity in horizontal cells but reduced the degree of staining in the neuronal processes of amacrine cells. MK-801 and N-methyl-D-aspartic acid (NMDA) had no effect on
NADPH diaphorase
activity in horizontal cells. However, MK-801 reduced staining in the neuronal processes of amacrine cells but not in their cell bodies. NMDA effects were expressed in a significant reduction in the number and size of amacrine cells that were
NADPH diaphorase
positive. These results indicate that activation of
NADPH diaphorase
in horizontal cells by darkness is mediated by the activation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainic acid (KA)-type glutamate receptors. The ON pathway in the retina is probably involved in modulation of
NADPH diaphorase
in the neuronal processes of amacrine cells. Amacrine cells that are
NADPH diaphorase
positive contain NMDA-type and AMPA/KA-type receptors and are highly susceptible to NMDA and kainic acid toxicity.
...
PMID:NADPH diaphorase activity in the rabbit retina is modulated by glutamatergic pathways. 1116 88
