Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.6.99.1 (NADPH-diaphorase)
 3,903 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cysteamine (100 micrograms) markedly reduces the number (by about 60%) and intensity of staining of NADPH diaphorase-reactive neurons 6 h after local injection into the striatum. This effect was reversible (after 24 h) and was only observed when the indirect staining procedure was applied in which NADPH formed by endogenous malate dehydrogenase is used. However, no direct effect of cysteamine on the malate dehydrogenase reaction was found. The decrease in NADPH diaphorase activity parallels the previously reported cysteamine induced decrease in somatostatin contained in the same neurons and may point to a biochemical interrelation of somatostatin and NADPH diaphorase in these neurons.
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PMID:Local injection of cysteamine into the rat striatum decreases number and intensity of staining of neurons by indirect NADPH diaphorase reaction. 245 Mar 8

Blockade of nitric oxide synthase (NOS) activity in the developing nervous system may protect the brain from hypoxic-ischemic insult. We determined the efficacy in 7 day old rat pups of systemically administered cysteamine in reducing neuronal NOS and nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase reactivities and protection of the brain from an hypoxic-ischemic insult. Cysteamine reversibly reduced NOS immunoreactivity at 2 h after an intraperitoneal injection of 200 mg/kg. NADPH-diaphorase histochemical reactivity was reduced after 300 mg/kg but all animals had generalized seizures and succumbed to the hypoxia-ischemia. At lower doses, despite the blockade of NOS immunoreactivity, there was no difference in the number of injured animals compared to controls. These results demonstrate that NOS immunoreactivity does not represent all of NADPH-diaphorase reactivity and that blockade of this activity with cysteamine is not protective.
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PMID:Cysteamine eliminates nitric oxide synthase activity but is not protective to the hypoxic-ischemic neonatal rat brain. 884 8