Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.6.99.1 (NADPH-diaphorase)
 3,903 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The investigation was carried out on 86 white non-inbred male rats. Hypokinesia was produced by putting the animals into pencil-boxes for 1,2 and 4 weeks. The changes in specific volumes of various hepatic microvessels under hypokinesia and under hypokinesia combined with metyrapone administration were studied sterologically. NAD-diaphorase activity in hepatocytes and in the interlobular connective tissue was estimated photometrically. hypokinesia produces certain changes in the specific volume of all the hepatic microvessels studied, but the degree and character of the volumetric relations revealed between these microvessels are different. At metyrapone administration, the volumetric changes in various microvessels occur with greater regularity. The changes in NAD-diaphorase activity under the same conditions occur slower and by the end of the experiment the enzymatic activity returns to the initial level.
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PMID:[Functional and morphological characteristics of rat liver microvessels in hypokinesia]. 709 90

Previous animal studies have demonstrated that systemic administration of 3-nitropropionic acid (3-NP) leads to neuropathological changes similar to those seen in Huntington's disease (HD). Recently, we reported hypoactivity in 6- and 10-week old rats treated with systemic 3-NP (IP, 10 mg/kg/day) once every 4 days for 28 days. Although these behavioral results seem to differ from the observed hyperactivity in most excitotoxic models of HD, 3-NP may provide a better model of juvenile onset and advanced HD. In the present study, older rats were similarly treated with 3-NP to further characterize the reported age dependency of striatal neuronal death caused by 3-NP. Hypoactivity was observed in 14- and 28-week old rats with the latter demonstrating more profound features. The present study also provided the first direct evidence of a 3-NP effect on passive avoidance behavior. Experimental and control animals showed no significant difference in daytime acquisition and retention of a passive avoidance task. However, when the retention tests were conducted during the night time (in contrast to previous daytime tests), 3-NP-treated animals exhibited significant retention deficits. In addition, the neuropathological effects of 3-NP were determined by Nissl, AChE and NADPH-diaphorase histochemistry. Metabolic activity was studied using cytochrome oxidase activity as an index. Results revealed striatal glial infiltration, loss of intrinsic striatal cholinergic neurons, but some sparing of large AChE positive neurons, minimal damage of NADPH-diaphorase-containing neurons, and very slight, if any, alterations in cytochrome oxidase activity.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Systemic 3-nitropropionic acid: behavioral deficits and striatal damage in adult rats. 753 73