Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.6.99.1 (NADPH-diaphorase)
 3,903 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The genetic structure of two Chukot Evens subpopulations (314 individuals) for electrophoretic protein systems and taste sensitivity to PTC was studied. 17 of the 39 loci were polymorphic (43.59%). The following systems were completely monomorphic: diaphorase NAD H (Dia); glucose-6-phosphate dehydrogenase (G-6-PD); glutamatoxalate transaminase (GOT); carbonic anhydrase (Ca-1); catalase (Ct), lactate dehydrogenase (loci LDH-A and LDH-B); leucine aminopeptidase (Lap); malate dehydrogenase (MDH); purine nucleoside phosphorylase (PNP); superoxide phosphorylase (PNP); superoxide dismutase (SOD); phosphoglucomutase-2 (PGM2); cholinesterase (locus E1); red cell esterase (4 loci); albumin (Alb); hemoglobin (Hb A and B); ceruloplasmin (Cp); and blood, gren, using the standard method. The following systems were polymorphic: red cell acid phosphatase (AcP); phosphoglucomutase-1 (PGM1); 6-phosphogluconate dehydrogenase (PGD); glutamatepyruvate transaminase (GPT); glyoxalase-1 (GLO-1); esterase (EsD); adenilatkinase (AK); alkaline phosphatase (Pp); cholinesterase (locus E2); haptoglobin (Hp); transferrin (Tf); group-specific component (Gc) and ABO, MN, Lewis, P blood groups and taste sensitivity to PTC. The following allele frequencies for polymorphic loci have been detected: AKI = 0.994; GLO = 1I = 0.082; GPT1 = 0.653; AcPA = 0.400; AcPB = 0.599; AcPC = 0.001; PGDA = 0.944; PGM1(1) = 0.906; EsD1 = 0.897; E2+ = 0.048; HpI = 0.394; GcI = 0,919; Tfc = 0.987; r(O) = 0.669; p(A) = 0.184; q(B) = 0.146; M = 0.711; Le = 0.411; P1+ = 0.521; t = 0.295. The genetic structure of Chukot Evens population is significantly nearer to that of the other ethnic groups of the North-East, in comparison with the genetic structure of Evenks of the Middle Siberia.
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PMID:[Genetic structure of the populations of native inhabitants in the northeastern USSR. V. The Chukot Evens]. 293 99

Using an in vitro system, the growth characteristics and enzyme histochemical properties of 3 odontogenic keratocysts and 3 dentigerous cysts were studied. It was found that the epithelial cells of the keratocysts but not of the dentigerous cysts grew in vitro. Furthermore, the epithelial-like cells of the keratocysts showed the same activities of acid phosphatase and NADH-diaphorase in vitro as earlier described in vivo. These enzymatic activities were increased in epithelial-like cells close to proliferating fibroblast-like cells, indicating a close relationship between these two cell types. The results are discussed in the light of the known clinical behaviour of the keratocyst and certain conclusions are also drawn concerning the suggested neoplastic potential of the keratocyst.
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PMID:In vitro growth characteristics of human odontogenic keratocysts and dentigerous cysts. 308 37

Osteogenesis of the body of the mandible in embryonic and neonatal rats was studied histologically and by histochemistry to determine the role of Meckel's cartilage in bone formation. Meckel's cartilage showed intense activity of lactate dehydrogenase and NADH2-diaphorase and weak activity of acid phosphatase, indicating a functioning citric acid cycle, pentose phosphate shunt and a capacity for anaerobic metabolism. The activity of these enzymes declined after hypertrophy of Meckel's cartilage. Alkaline phosphatase was the major enzyme of mineralising mandibular osteoid and was present in the osteoblasts and osteoprogenitor cells but not in Meckel's cartilage. After the differentiation of Meckel's cartilage and intramembranous bone, Meckel's cartilage supported mandibular bone formation by endochondral ossification in the anterior part of the mandible.
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PMID:Enzyme histochemical analysis of Meckel's cartilage. 325 49

The effects of irradiation on various tissues have been studied extensively. Nonetheless, the metabolism in growing bones has not been evaluated in a systematic way after moderate doses of irradiation. It was found that scattered radiation, that reaches the oral region during radiotherapy of malignancies outside the oral region, causes absorbed doses within the range of 0.2-20 Gy, while absorbed doses from radiography in orthodontics were only 30-40 mGy. Bone formation in the metaphyseal area of rat tibia in vivo after irradiation with 0.5-8 Gy was determined by a tetracycline labelling method. Five and 8 Gy induced a significant growth retardation. This was detectable already after 36 hours and was maximal 7-14 days after irradiation. Between 14 and 30 days following irradiation growth was normalized. Alkaline phosphatase (ALP) activity in bone was evaluated biochemically and decreased one day after irradiation with 0.5-8 Gy. This was followed by a gradual increase in ALP activity and a return to normal values 30 days after irradiation. Histochemical studies of the rat tibias included evaluation of ALP, acid phosphatase, NADH2-diaphorase and Glucose-6 phosphate dehydrogenase. A decrease in ALP activity one day after irradiation was observed with 5, 8, and 10 Gy. Acid phosphatase and the two oxidative enzymes were increased in activity during the entire 7-day experimental period, reflecting an altered metabolism. Normal activities of all the studied enzymes were observed 30 days after irradiation. Results from suture area and synchondrosis area as evaluated by histochemistry and a cephalometric radiographic method showed that early transient metabolic changes occurred in the craniofacial growth sites after irradiation with 5 and 8 Gy. The morphological changes observed in anatomical regions within the irradiated field (neurocranium) persisted in contrast to the changes in the viscerocranium that were normalized at the end of the experimental period. An in vitro system was used to examine the effects of irradiation on certain aspects of bone growth. Mice calvaria were irradiated in vitro with 2 or 10 Gy. A different response in suture and bone was found 3 hours to 4 days after irradiation. Bone was affected by 2 Gy, but not the suture. Thus, the suture seems to be an area with more radioresistant fibroblast-like cells than the cortical bone, which indicates a difference in radiosensitivity of the cells in these two growth sites. The conclusions from the present thesis are that irradiation with 2-10 Gy of bone both in organ culture and in experimental animals induces metabolic and morphologic changes which were detected early and were transient.
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PMID:Effects of irradiation on growing bones. 346 72

Histochemical investigations were carried out to demonstrate the activity of succinic dehydrogenase, diphosphopyridine-nucleotid-diaphorase, alkaline phosphatase, and acid phosphatase in the liver, kidneys, lymph nodes, and heart muscle of a total of 20 cows with positive serologic response for leukosis as well as in organs of 2 normal controls. It was found that the lymphoid cells of the leukotic proliferations and the activated endothelial and adventitial cells of the blood vessels had high alkaline phosphatase activity and negligibly expressed acid phosphatase activity. Dehydrogenase activity was low in the lymphoid-cell proliferations and in the activated cell of the vessels. The cell metabolism of the leukotic proliferations was like-wise disturbed. Histochemical methods of investigations could be used test-like in the diagnosis of bovine leukosis.
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PMID:[Histochemical changes in leukemia in cattle]. 399 26

The enzyme pattern of 13 cases of malignant fibrous histiocytoma (MFH) and 11 cases of myxofibrosarcoma (MFS), a malignant myxomatous soft tissue tumor of fibroblastic histiocytic origin, has been studied. 6 of the 13 MFHs were analyzed enzyme histochemically at the light microscopic level and 7 on the ultrastructural level; of the 11 MFSs 9 were analyzed enzyme histochemically at the light microscopic level and 2 on the ultrastructural level. Differences were observed in the subjectively estimated enzyme activity between low grade MFS and high grade MFS and MFH, and also between histiocyte-like and fibroblast-like tumor cells. Generally a strong reaction of oxidoreductase enzymes (NADH2-diaphorase, NADPH2-diaphorase, glucose-6-phosphate dehydrogenase) and hydrolytic enzymes (acid phosphatase and leucine aminopeptidase) was found in the high grade tumors and was usually higher in the histiocyte-like than in the fibroblast-like cells. Ultrastructurally acid phosphatase occurred predominantly in primary and secondary lysosomes and Golgi zones of the histiocyte-like cells. A strong reaction of alkaline phosphatase was found light microscopically in 2 of 5 MFHs and 5 of 9 MFSs. Ultrastructurally alkaline phosphatase was located along the cytoplasmic membrane of predominantly fibroblast-like cells in 3 of 7 MFHs and 1 of 2 MFSs. The results agree with the concept of two main cell types in MFH and MFS, fibroblasts and histiocytes.
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PMID:Enzyme histochemistry of malignant fibroblastic histiocytic tumors. A light and electron microscopic analysis. 608 56

Quantitative cytochemical investigations have detected individual variations between murine peritoneal macrophages and have shown distinct difference between resident and exudate populations. The latter generally contain greater amounts of protein, RNA, acid phosphatase, succinate dehydrogenase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, and NADH dehydrogenase. On te other hand, no differences were detected in the cellular content of DNA, not-specific esterase, and NADPH dehydrogenase. In many instances they reflect the biochemical findings of other investigators including the stimulation of glycolysis, tricarboxylic acid cycle and hexose monophosphate shunt pathways, which can occur in elicited or activated macrophages. Although cytochemical differences between the two populations exist, it cannot be stated whether they represent distinct cell lines or different functional states of the same cell population.
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PMID:A quantitative cytochemical analysis of resident and exudate macrophages. 616 17

Oral administration of manganese chloride (25 mg/kg b. w. daily) to monkeys for a period of 18 months produced congestion and marked increase in weight of testis. Histopathologic examination revealed interstitial oedema and degeneration of seminiferous tubules. Activities of succinic dehydrogenase, glucose-6-phosphate dehydrogenase and acid phosphatase were significantly inhibited whereas NADH-diaphorase and alkaline phosphatase activities showed only slight inhibition in seminiferous tubules of treated monkeys. It was concluded that chronic exposure to manganese does not produce sever degenerative changes in the testis earlier than metal induced encephalopathy in primates.
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PMID:Manganese induced testicular changes in monkeys. 624 33

Oxygen and glucose consumption and lactate production of the peritoneal membrane and intra-abdominal adhesions were measured in rats after a single intra-peritoneal colloidal silica injection. Enzyme histochemical studies were made of lactate dehydrogenase, succinate dehydrogenase, NADH2-diaphorase, NADPH2-diaphorase, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, acid phosphatase, leucylaminopeptidase and alkaline phosphatase in the peritoneal membrane. Anaerobic glycolysis comprises 47% of the total glucose consumption in the the normal peritoneum. Glucose consumption and lactate production of the peritoneal membrane increased sharply in the early phase of silica-induced peritonitis and stayed at a high level for a week indicating an enhanced anerobic metabolism. Oxygen and aerobic glucose consumption increased more slowly than anaerobic glucose consumption and reached their maxima 1 week after silica injection, indicating that the rate of aerobic metabolism is also higher in chemical peritonitis than in the controls. On the other hand, glucose consumption and lactate production increased in a parallel fashion in adhesions and in the peritoneum in the early phase of peritonitis. However, the maximum and later levels were less in adhesions than in the peritoneum. In the enzyme histochemical study high activities of enzymes indicating anaerobic energy metabolism and metabolism via the pentose phosphate shunt were seen in cells of the peritoneal membrane during the early phase of peritonitis. No activity was identified in enzymes indicating aerobic energy metabolism and increased catabolism before the end of the first week.
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PMID:Energy metabolism of the peritoneal membrane in silica-induced peritonitis. A biochemical and enzyme histochemical study. 625 64

The activity of some dehydrogenases and hydrolases was studied by cytochemical methods in the peripheral blood neutrophils of germ-free guinea pigs infected with adenoviruses. The gnotobiotic animals were obtained by hysterotomy in an operation isolation room after which they were transferred into manipulation isolation room and infected with human adenovirus type 1. A depression of enzymes of alpha-glycerophosphate shunt and NADP-H2-diaphorase in neutrophils two days after infection and activation of lactate dehydrogenase and acid phosphatase at 4 days were demonstrated. The pattern of changes in the enzymatic status of intact and infected gnotobiotic animals allowed a diagnosis of adenovirus infection in most cases.
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PMID:[Cytochemical study of granulocyte enzymes in germ-free animals with adenovirus infections]. 626 24


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