Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.6.5.4 (
SOR
)
720
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Nuclear protein
which selectively binds to the Alu-family DNA repeat (
AFR
, Blur8) is partially purified from human HeLa cells using a gel retention assay. At low protein concentrations only a single complex of the protein with
AFR
is formed (CII). Increasing protein concentrations lead to the gradual disappearance of CII, being replaced by complexes with higher (CI) and lower (CIII, CIV) electrophoretic mobilities. Differential binding of
AFR
restriction subfragments indicates that multiple protein-binding sites are present within
AFR
. We discuss two models explaining the anomalous electrophoretic mobility of CII by DNA bending or looping upon cooperative multi-site binding of the protein to
AFR
.
...
PMID:Multi-site binding of human nuclear protein to the Alu-family repeated DNA. 283 72
