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Query: EC:1.6.5.4 (
SOR
)
720
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In this paper, we investigated the relationship between hydrogen sulfide (H
2
S) and mitogen-activated protein kinase kinase (
MEK1
/2) in jasmonic acid (JA)-regulated the redox state of ascorbate in the leaves of
Arabidopsis thaliana
. The results showed that JA significantly enhanced the phosphorylation level of
MEK1
/2, the production of endogenous H
2
S and the ratio of reduced ascorbate (AsA) to dehydroascorbate (DHA) (AsA/DHA) in wild type of
A. thaliana
(WT). However, there were no obvious effects of JA on above indicators in H
2
S synthetic mutant of
A. thaliana
(MT). H
2
S scavenger hypotaurine (HT) markedly reduced JA-induced the phosphorylation level of
MEK1
/2, AsA/DHA ratio and the production of endogenous H
2
S in WT. Application of H
2
S donor sodium hydrosulfide (NaHS) to JA-treated MT plants increased above indicators. Application of NaHS to (HT+JA)-treated MT plants did not reverse the effects of HT on above JA-induced indicators.
MEK1
/2 inhibitor PD98059 decreased JA-induced AsA/DHA ratio and the transcript levels and the activities of ascorbate peroxidase (APX), glutathione reductase (GR),
monodehydroascorbate reductase
(MDHAR), dehydroascorbate reductase (DHAR) and L-galactono-1,4-lactone dehydrogenase (GalLDH) in WT. However, PD98059 had no effect on JA-induced the production of endogenous H
2
S in WT. Compared with Control-MT, there were no obvious effects of JA on the production of endogenous H
2
S, AsA/DHA ratio and the transcript levels and activities of above enzymes in MT. However, application of PD98059 reduced above JA-induced indicators except the production of endogenous H
2
S and DHA content in MT. Our results suggested that H
2
S activated
MEK1
/2 in JA-regulated AsA/DHA ratio in
A. thaliana
leaves through enzymes in ascorbate metabolism.
...
PMID:Jasmonic acid-induced hydrogen sulfide activates MEK1/2 in regulating the redox state of ascorbate in
Arabidopsis thaliana
leaves. 3118 85
