Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.6.5.4 (SOR)
 720 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Ascorbate is the most abundant small molecule antioxidant in plants and is proposed to function, along with other members of an antioxidant network, in controlling reactive oxygen species. A biochemical and molecular characterization of four ascorbate-deficient (vtc) Arabidopsis thaliana mutants has been carried out to determine if ascorbate deficiency is compensated by changes in the other major antioxidants. Seedlings grown in vitro were used to minimize stress and longer term developmental differences. Comparison was made with the low glutathione cad2 mutant and vtc2-1 treated with D,L-buthionine-[S,R]-sulphoximine to cause combined ascorbate and glutathione deficiency. The pool sizes and oxidation state of ascorbate and glutathione were not altered by deficiency of the other. alpha-Tocopherol and activities of monodehydroascorbate reductase, dehydroascorbate reductase, glutathione reductase, and catalase were little affected. Ascorbate peroxidase activity was higher in vtc1, vtc2-1, and vtc2-2. Ionically bound cell wall peroxidase activity was increased in vtc1, vtc2-1, and vtc4. Supplementation with ascorbate increased cell wall peroxidase activity. 2,6-Dichlorobenzonitrile, an inhibitor of cellulose synthesis, increased cell wall peroxidase activity in the wild type and vtc1. The transcript level of an endochitinase, PR1, and PR2, but not GST6, was increased in vtc1, vtc2-1, and vtc-2-2. Endochitinase transcript levels increased after ascorbate, paraquat, salicylic acid, and UV-C treatment, PR1 after salicylic acid treatment, and PR2 after paraquat and UV-C treatment. Camalexin was higher in vtc1 and the vtc2 alleles. Induction of PR genes, cell wall peroxidase activity, and camalexin in vtc1, vtc2-1, and vtc2-2 suggests that the mutants are affected in pathogen response signalling pathways.
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PMID:Antioxidant status, peroxidase activity, and PR protein transcript levels in ascorbate-deficient Arabidopsis thaliana vtc mutants. 1884 95

Senescence is a developmentally regulated and highly ordered sequence of events. Senescence leads to abscission of plant organs and eventually leads to death of a plant or part of it. Present study revealed that Phalaenopsis flower undergo senescence due to over activation of O(2) (.-)generating xanthine oxidase (XO), which consequently increases the concentrations of O(2) (.-) leading to enhanced oxidative damage and disturbed cellular redox environment as indicated by increased lipid peroxidation and DHA/AsA + DHA ratio, respectively. While activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and non-specific peroxidase (POD) were enhanced in sepals and petals of old flower, activities of catalase (CAT) and glutathione reductase (GR) were decreased. Exogenous application of nitric oxide (NO) retarded H(2)O(2)-induced senescence of Phalaenopsis flower by downregulating activity of XO and concentrations of O(2) (.-), H(2)O(2) and malondialdehyde (MDA, an index of lipid peroxidation). Exogenous application of NO also downregulated SOD activity and upregulated antioxidant enzymes involved in the detoxification of H(2)O(2) (CAT and APX), and in the regulation of redox couples viz, monodehydroascorbate reductase (MDHAR) and GR, together with the modulation in non-protein thiol status and DHA/AsA + DHA ratio.
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PMID:Nitric oxide retards xanthine oxidase-mediated superoxide anion generation in Phalaenopsis flower: an implication of NO in the senescence and oxidative stress regulation. 1898 52

Dunaliella species accumulate carotenoids and their role in protection against photooxidative stress has been investigated extensively. By contrast, the role of other antioxidants in this alga, has received less attention. Therefore, the components of the ascorbate-glutathione cycle, along with superoxide dismutase (E.C. 1.15.1.1) and peroxidase (E.C. 1.11.1.11) activity were compared in two strains of Dunaliella salina. Strain IR-1 had two-fold higher chlorophyll and beta-carotene concentration than Gh-U. IR-1 had around four-fold higher superoxide dismutase, ascorbate peroxidase and pyrogallol peroxidase activities than Gh-U on a protein basis. Ascorbate and glutathione concentrations and redox state did not differ between strains and there was little difference in the activity of ascorbate-glutathione cycle enzymes (monodehydroascorbate reductase [E.C. 1.6.5.4], dehydroascorbate reductase [E.C. 1.8.5.1] and glutathione reductase [E.C. 1.8.1.7]). The response of these antioxidants to high light and low temperature was assessed by transferring cells from normal growth conditions (28 degrees C, photon flux density of 100 micromol m(-2) s(-1))to 28 degrees C/1200 micromol m(-2) s(-1); 13 degrees C/100 micromol m(-2) s(-1); 13 degrees C/1200 micromol m(-2) s(-1) and 28 degrees C/100 micromol m(-2) s(-1) for 24 h. Low temperature and combined high light-low temperature decreased chlorophyll and beta-carotene in both strains indicating that these treatments cause photooxidative stress. High light, low temperature and combined high light-low temperature treatments increased the total ascorbate pool by 10-50% and the total glutathione pool by 20-100% with no consistent effect on their redox state. Activities of ascorbate-glutathione cycle enzymes were not greatly affected but all the treatments increased superoxide dismutase activity. It is concluded that D. salina can partially adjust to photooxidative conditions by increasing superoxide dismutase activity, ascorbate and glutathione.
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PMID:The effect of acute high light and low temperature stresses on the ascorbate-glutathione cycle and superoxide dismutase activity in two Dunaliella salina strains. 1923 61

Apple replant is a widespread agricultural problem documented in all of the major fruit-growing regions of the world. In order to better understand the phytotoxic mechanisms induced by allelochemicals involved with this problem, Malus prunifolia plants were grown hydroponically to the six-leaf-stage in the presence of phthalic acid (0 or 1 mM) for 5, 10, or 15 days. Apple plants were evaluated for: shoot and root length, fresh and dry weight, malondialdehyde (MDA) content, hydrogen peroxide (H(2)O(2)) content, superoxide radical (O(2) (*-)) generation rate, and antioxidant enzyme activities. Shoot and root lengths and fresh and dry weights of M. prunifolia decreased in plants exposed to phthalic acid. MDA and H(2)O(2) content increased in phthalic acid-treated plants as did the generation rate of O(2) (*-) in M. prunifolia roots. The activities of superoxide dismutase (EC 1.15.1.1), peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6), ascorbate peroxidase (EC 1.11.1.11), glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1), and monodehydroascorbate reductase (EC 1.6.5.4) increased in phthalic acid-stressed roots compared with control roots. These results suggest that activation of the antioxidant system by phthalic acid led to the formation of reactive oxygen species that resulted in cellular damage and the decrease of M. prunifolia growth.
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PMID:Phthalic acid induces oxidative stress and alters the activity of some antioxidant enzymes in roots of Malus prunifolia. 1935 74

Removal of reproductive 'sink' i.e. spikelets from wheat at anthesis delays the rate of flag leaf senescence. In this work, the antioxidant defense was studied in the flag leaf of Triticum aestivum cv. Kalyansona plants showing normal (S + plants) and delayed senescence via removal of spikelets (S- plants). This was done by measurement of metabolites and activities of enzymes such as superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase. S- plants had higher reduced glutathione/oxidized glutathione (GSH/GSSG) ratio and antioxidant enzyme activities than the control plants and the differences were apparent from 21 days after anthesis (DAA). The removal of the reproductive sink led to an increased antioxidant defense which may be contributing towards the delayed flag leaf senescence in wheat. Chloroplasts and mitochondria, important sources of ROS, were isolated at two stages representing early (7 DAA) and late (21 DAA) senescence. Oxidative damage to proteins was studied in these organelles in relation to SOD and APX. Mitochondria had higher levels of damaged proteins than chloroplasts at 7 DAA in both S+ and S- plants. Higher damage was related to the lower antioxidant enzyme levels of SOD and APX in mitochondria as compared to chloroplasts.
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PMID:Delayed wheat flag leaf senescence due to removal of spikelets is associated with increased activities of leaf antioxidant enzymes, reduced glutathione/oxidized glutathione ratio and oxidative damage to mitochondrial proteins. 1939 42

The effect of different cadmium (Cd) concentrations (5, 50 and 500 microM) on growth, Cd accumulation and antioxidative systems was studied in Paxillus involutus, grown in liquid medium. Cd was rapidly accumulated by P. involutus and resulted in growth inhibition within 24 h. Antioxidative enzymes (superoxide dismutase (SOD), EC 1.15.1.1; catalase (CAT), EC 1.11.1.6; monodehydroascorbate radical reductase (MDAR), EC 1.6.5.4; dehydroascorbate reductase (DAR) glutathione reductase (GR), EC 1.8.1.7 and glutathione-dependent peroxidase (GPx), EC 1.11.1.9) were active in the investigated fungus. Furthermore, high concentrations of glutathione but no ascorbate were detected. Cd exposure resulted in a significant induction of SOD activity. However, activities of enzymes responsible for the detoxification of H2O2 showed no Cd-dependent increase or were only transiently induced (CAT, GPx) and no accumulation of H2O2 was detected. Exposure to low Cd concentrations (5 and 50 microM) caused an increase in GR, while 500 microM Cd led to an inhibition of GR and CAT. Increased glutathione concentrations were observed as a consequence of all Cd treatments. These results suggest that the antioxidative protection of the investigated strain of P. involutus was sufficient to avoid Cd-mediated oxidative stress. It is likely that this strain was able to detoxify high concentrations of Cd by transport of Cd into the vacuole because a high correlation between Cd and sulphur in the vacuole was detected by EDX.
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PMID:Characterisation of antioxidative systems in the ectomycorrhiza-building basidiomycete Paxillus involutus (Bartsch) Fr. and its reaction to cadmium. 1970 95

Plants have evolved mechanisms to avoid and repair UV radiation damage, and the free radicals caused by UV tend to be involved in the induction of antioxidant defense systems. In this study, changes in resveratrol and antioxidant enzymes were investigated in relation to UV damage in peanut seedlings. Accumulation of endogenous resveratrol and stilbene synthase mRNA occurred rapidly and significantly in response to UV-C irradiation. Applying resveratrol before UV-C irradiation mitigated rusty spots and wilting of peanut leaves, and inhibition of resveratrol by applying 3,4-methylenedioxycinnamic acid worsened UV-C damage, an effect that was found to be concentration dependent. Correspondingly, the effect of resveratrol on malondialdehyde was similar to changes in the apparent morphology of seedling leaves. Changes in H(2)O(2), O(2)(-), and antioxidant enzymes showed some similarities after either UV-C irradiation or resveratrol treatment. Activities of superoxide dismutases, glutathione reductase, and catalase were more than 2-fold higher during the first 1h after treatments. Ascorbate peroxidase activity increased to more than 3-fold higher 24h after irradiation, whereas it was more than 2-fold higher 8h after resveratrol treatment. Activities of dehydroascorbate reductase and monodehydroascorbate reductase increased by 40% during 8-24h after treatments. Consequently, we proposed that changes in endogenous resveratrol and in antioxidant enzymes may have been involved in oxidative stress induced by UV-C exposure in peanut seedlings.
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PMID:Changes of resveratrol and antioxidant enzymes during UV-induced plant defense response in peanut seedlings. 1971 23

Spartina densiflora is an invasive cordgrass that is colonizing new habitats and ousting indigenous species in pro-oxidative environments like cadmium-polluted salt marshes in the Odiel estuary (Spain). The aim of our study was to characterize its antioxidative system in order to find out if the system underlies the tolerance of S. densiflora to cadmium toxicity. S. densiflora plants were firstly evaluated to ascertain its antioxidative status in the natural habitat and then they were cultured in the laboratory in unpolluted sand for 28 days. Throughout this period, plants acclimatized and oxidative stress markers reached stable low levels. Then, S. densiflora plants were exposed to cadmium concentrations (10, 100 and 1000 microM Cd) for another 28 days. Higher Cd content in leaves was related to higher level of reactive oxygen species (ROS) causing important oxidative cell damage (lipid peroxidation and lower chlorophyll content). However, S. densiflora possesses a well-organized and appropriately modulated antioxidative defense system which comprises enzymatic activities of guaiacol peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6), ascorbate peroxidase (EC 1.11.1.11) and superoxide dismutase (EC 1.15.1.1) coupled with the activation of the ascorbate cycle, including enzymatic activities of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and monodehydroascorbate reductase (EC 1.6.5.4). This activation was sufficient to reduce Cd-induced ROS accumulation and oxidative damage caused by the lowest Cd-concentrations, but not by the highest Cd-concentration (1000 microM). Nevertheless, the antioxidant system seems to be efficient to achieve a tolerance to cadmium toxicity, allowing normal plant development, even at the presence of highest Cd concentration.
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PMID:Cadmium-induced oxidative stress and the response of the antioxidative defense system in Spartina densiflora. 2021 Aug 72

The effect of acidified calcium sulfate (ACS) on the quality of litchi ( Litchi chinensis Sonn. cv. 'Brewster') fruit after harvest was evaluated. ACS at 1.25% or higher concentrations significantly inhibited the activities of polyphenol oxidase and peroxidase in the pericarp during storage at both 5 and 10 degrees C. These treatments also effectively prevented browning and retained the red color of the outer shell of the fruit. Total phenolic and total anthocyanin contents in pericarp were increased by ACS treatments in a dose-dependent manner. The radical scavenging activities for ROO(*), DPPH(*), (*)OH and O(2)(*-) were also enhanced by ACS, particularly by 2.5 and 5% concentrations. The activities of several antioxidant enzymes and enzymes of ascorbate-glutathione cycle including catalase, ascorbate peroxidase, glutathione peroxidase, glutathione reductase, dehydroascorbate reductase, and monodehydroascorbate reductase gradually declined during storage. However, ACS enhanced the activities of these enzymes, especially at the beginning of the storage. Samples treated with ACS generally had higher flavonoid levels than the control. The three major flavonoids, cyanidin-3-rutinoside, cyanidin-3-glucoside and quercetin-3-rutinoside, were found to be significantly increased by 2.5 and 5.0% ACS at both 5 and 10 degrees C. No differences were detected among various treatments in soluble solids content or sugar and organic acid levels in the pulp of litchi fruit, indicating that the internal quality of the fruit was not adversely affected by ACS treatment.
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PMID:Maintaining quality of litchi fruit with acidified calcium sulfate. 2061 5

Ascorbate peroxidase (APX) of the liverwort Pallavicinia lyelli was extracted and purified through ammonium sulfate precipitation, Butyl-Toyopearl, DEAE-Cellulofine and Sephadex G-75 chromatography. The purification factor for APX was 285 with 7.9% yield. The enzyme was characterized for thermal stability, pH and kinetic parameters. The molecular mass of APX was approximately 28 kDa estimated by SDS-PAGE. The purity was checked by native PAGE, showing a single prominent band. The optimum pH was 6.0. The enzyme had a temperature optimum at 40 degrees C and was relatively stable at 60 degrees C, with 54% loss of activity. When the enzyme was diluted with the ascorbate-deleted medium, the half inactivation time was approximately 15 min. The absorption spectra of the purified enzyme and the inhibition by cyanide and azide showed that it is a hemoprotein. Spectral analysis and inhibitor studies were consistent with the presence of a heme moiety. When compared with ascorbate peroxidase activity derived from ruptured intact chloroplasts, the purified enzyme was found to have a higher stability, a broader pH optimum for activity and the capacity to utilize alternate electron donors. p-Chloromercuribenzoate (pCMB), hydroxyurea and salicylic acid (SA) significantly inhibited APX activity. Ascorbate (AsA) and pyrogallol were found to be efficient substrates for Pallavicinia APX, considering the Vmax/Km ratio. We detected the activity of monodehydroascorbate reductase (MDHAR) involved in the regeneration of ascorbate, but failed to detect the dehydroascorbate reductase (DHAR) activity. The data obtained in this study may help to understand desiccation tolerance mechanism in the liverwort.
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PMID:Purification and kinetic characterization of the liverwort Pallavicinia lyelli (Hook.) S. Gray. cytosolic ascorbate peroxidase. 2061 66


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