Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.6.5.4 (
SOR
)
720
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Dioscorin, the major tuber storage protein of yam, has been shown to possess carbonic anhydrase, trypsin inhibitor, dehydroascorbate reductase, and
monodehydroascorbate reductase
activities. In the present study, dioscorin from Dioscorea japonica was confirmed as a
glycoprotein
using the enhanced concanavalin A-peroxidase staining method, and the protein was shown to have both N- and O-glycans. Following the gene cloning, four full-length isoforms of dioscorin were expressed in Escherichia coli and purified by affinity purification and anion-exchange chromatography for structural and biochemical experiments. It was clearly observed that the recombinant dioscorins had carbonic anhydrase, trypsin inhibitor, dehydroascorbate reductase, and
monodehydroascorbate reductase
activities. However, the dehydroascorbate reductase and
monodehydroascorbate reductase
activities were markedly decreased in recombinant dioscorins compared with native dioscorin. The decreased activities were closely related to the loss of the glycosylation from the protein.
...
PMID:Cloning of genes and enzymatic characterizations of novel dioscorin isoforms from Dioscorea japonica. 2219 72
