Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.6.5.3 (
complex I
)
8,901
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The type I signal peptidase lepB genes from Rickettsia rickettsii and Rickettsia typhi, the etiologic agents of Rocky Mountain spotted fever and murine typhus, respectively, were cloned and characterized. Sequence analysis of the cloned lepB genes from R. rickettsii and R. typhi shows open reading frames of 801 and 795 nucleotides, respectively. Alignment analysis of the deduced amino acid sequences reveals the presence of highly conserved motifs that are important for the catalytic activity of bacterial type I signal peptidase. Reverse transcription-PCR and Northern blot analysis demonstrated that the lepB gene of R. rickettsii is cotranscribed in a polycistronic message with the putative nuoF (encoding
NADH dehydrogenase
I chain F), secF (encoding protein export membrane protein), and rnc (encoding
RNase III
) genes in a secF-nuoF-lepB-rnc cluster. The cloned lepB genes from R. rickettsii and R. typhi have been demonstrated to possess signal peptidase I activity in Escherichia coli preprotein processing in vivo by complementation assay.
...
PMID:Molecular and functional analysis of the lepB gene, encoding a type I signal peptidase from Rickettsia rickettsii and Rickettsia typhi. 1286 68
Anoxygenic photosynthesis is an important pathway for
Rhodobacter sphaeroides
to produce ATP under oxygen-limiting conditions. The expression of its photosynthesis genes is tightly regulated at transcriptional and post-transcriptional levels in response to light and oxygen signals, to avoid photooxidative stress by the simultaneous presence of pigments, light and oxygen. The
puf
operon encodes pigment-binding proteins of the light-harvesting
complex I
(genes
pufB
and
pufA
), of the reaction center (genes
pufL
and
pufM
), a scaffold protein (gene
pufX
) and includes the gene for sRNA PcrX. Segmental differences in the stability of the
pufBALMX-pcrX
mRNA contribute to the stoichiometry of LHI to RC complexes. With asPcrL we identified the third sRNA and the first antisense RNA that is involved in balancing photosynthesis gene expression in
R. sphaeroides
. asPcrL influences the stability of the
pufBALMX-pcrX
mRNA but not of the
pufBA
mRNA and consequently the stoichiometry of photosynthetic complexes. By base pairing to the
pufL
region asPcrL promotes
RNase III
-dependent degradation of the
pufBALMX-prcX
mRNA. Since asPcrL is activated by the same protein regulators as the
puf
operon including PcrX it is part of an incoherent feed-forward loop that fine-tunes photosynthesis gene expression. [Figure: see text].
...
PMID:Antisense RNA asPcrL regulates expression of photosynthesis genes in
Rhodobacter sphaeroides
by promoting RNase III-dependent turn-over of
puf
mRNA. 3325 5
