Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.6.5.3 (
complex I
)
8,901
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In order to determine the site of
PAF
synthesis via the remodeling pathway in undifferentiated HL-60 cells, a subcellular fractionation procedure (Ribbes et al., 1985) was adapted to separate various membrane fractions and cellular organelles of HL-60 cells across a Percoll gradient. The profile of alkyllyso-GPC:acetyl-CoA acetyltransferase activity closely paralleled the subcellular distribution of the endoplasmic reticulum marker,
NADH dehydrogenase
. These data indicate the acetyltransferase in the remodeling pathway of
PAF
synthesis is located at the same cellular site as the acyltransferase(s) that catalyze the transfer of long-chain acyl moieties from acyl-CoAs to lyso-phospholipids. Also the acetyltransferase in undifferentiated HL-60 cells appears to have a similar intracellular distribution as reported for human neutrophils.
...
PMID:Intracellular location of acetyltransferase in the remodeling pathway of PAF biosynthesis in undifferentiated human leukemic cells (HL-60). 213 65
The subcellular distribution of an alkyllyso-GPC: acetyl-CoA acetyltransferase (EC 2.3.1.67) and transacylase, two important enzyme activities involved in the remodeling pathway for the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine,
PAF
) have been examined in leukocytes isolated from the pronephros of the rainbow trout, Oncorhynchus mykiss. Contrary to mammalian systems, in which the acetyltransferase is localized to intracellular membranes, the subcellular distribution of an acetyltransferase activity in rainbow trout leukocytes was localized to the plasma membrane. Analysis of the acetyltransferase products by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) confirmed synthesis of two subclasses of
PAF
, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine. The transacylase activity in this study was detected in membrane fractions in two domains of the intermediate density region which also contained the
NADH dehydrogenase
activity, a marker enzyme for the endoplasmic reticulum. Acylation of lysoPAF (1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine) exhibited approximately 95% specificity for omega-3 fatty acids. Acylation patterns were not significantly different in either domain of the endoplasmic reticulum. A model is proposed herein for the metabolism of
PAF
in rainbow trout leukocytes.
...
PMID:Subcellular localization of enzyme activities involved in the metabolism of platelet-activating factor in rainbow trout leukocytes. 1010 Dec 69
