Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.6.5.2 (NQO1)
 6,196 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Frozen sections of eight odontogenic cysts, including one keratocyst, were incubated to show the following enzyme activities: NADH2 diaphorase, NADPH2 diaphorase, glucose-6-phosphate dehydrogenase, acid phosphatase and leucine aminopeptidase. The disbribution of lipid was shown by the oil red 0 method. The activities of all three oxidative enzymes were strongest in epithelial cells bordering hyalin bodies and in basal cells in the epithelial lining. Hydrolytic enzyme activity was absent from all but the most superficial epithelial cells but was present in macrophages and, in lesser amounts, in granular material in the same sections. The granular material frequently contained lipid. The lack of hydrolytic enzyme activity in bordering epithelial cells is inconsistent with the theory that hyalin bodies form from degenerating blood vessels. High aerobic oxidative enzyme activity in the same cells also conflicts with the concept that the bodies are a keratinous product. The findings lend support to the theory that hyalin bodies are an epithelial secretion.
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PMID:Enzyme histochemical studies on the formation of hyalin bodies in the epithelium of odontogenic cysts. 5 1

Of a total of 1,420 odontogenic cysts, 52 (3.3%) were diagnosed as odontogenic keratocysts. Clinical and histological findings in these 52 cysts are reported. Frozen sections of 26 of the keratocysts were incubated to show the following enzyme activities: NADH2- and NADPH2-diaphorase, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, acid phosphatase, leucine aminopeptidase and ATPase. Furthermore, keratinization was studied with the rhodamine B method and lipids with the oil red O, the OTAN and the acid hematein methods. Sections from epidermis, oral mucosa, radicular cysts, residual cysts and follicular cysts served as reference material. The oxidative enzymes showed strong activity in the keratocyst epithelium which contrasted with weak activity in the reference cysts. Acid phosphatase activity was weak in all epithelia except that in keratocysts, which displayed a marked activity. In the fibrous capsule of the keratocyst a high activity of leucine aminopeptidase was recorded. This high activity contrasted with a weak activity in the reference material. The significance of the histochemical results in relation to the aggressive behavior of the keratocyst is discussed.
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PMID:Odontogenic keratocysts: a clinical and histological study with special reference to enzyme histochemistry. 14 97

A reduction in the content of neutral mucopolysaccharides in mucous cells of the neck, a slight decrease in the activity of succinate dehydrogenase and NAD-diaphorase in parietal cells, a decrease in the DNA synthesis rate, and an increase in the area of mitochondria and cristae were detected in the gastric mucosa of rats which were in a long-term space flight. In the small intestine, an increase in the activity of glucose-6-phosphate dehydrogenase and leucine aminopeptidase were found. Morphological changes in the liver consisted in infiltrative adiposity. A similar morphological picture was demonstrated in a synchronous experiment on the earth. These changes, however, were nonspecific and reversible (25 days after rehabilitation the picture did not differ from the animal house control).
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PMID:[Morphological changes in the digestive organs during prolonged space flight on the Kosmos-782 biosatellite]. 15

The genetic structure of two Chukot Evens subpopulations (314 individuals) for electrophoretic protein systems and taste sensitivity to PTC was studied. 17 of the 39 loci were polymorphic (43.59%). The following systems were completely monomorphic: diaphorase NAD H (Dia); glucose-6-phosphate dehydrogenase (G-6-PD); glutamatoxalate transaminase (GOT); carbonic anhydrase (Ca-1); catalase (Ct), lactate dehydrogenase (loci LDH-A and LDH-B); leucine aminopeptidase (Lap); malate dehydrogenase (MDH); purine nucleoside phosphorylase (PNP); superoxide phosphorylase (PNP); superoxide dismutase (SOD); phosphoglucomutase-2 (PGM2); cholinesterase (locus E1); red cell esterase (4 loci); albumin (Alb); hemoglobin (Hb A and B); ceruloplasmin (Cp); and blood, gren, using the standard method. The following systems were polymorphic: red cell acid phosphatase (AcP); phosphoglucomutase-1 (PGM1); 6-phosphogluconate dehydrogenase (PGD); glutamatepyruvate transaminase (GPT); glyoxalase-1 (GLO-1); esterase (EsD); adenilatkinase (AK); alkaline phosphatase (Pp); cholinesterase (locus E2); haptoglobin (Hp); transferrin (Tf); group-specific component (Gc) and ABO, MN, Lewis, P blood groups and taste sensitivity to PTC. The following allele frequencies for polymorphic loci have been detected: AKI = 0.994; GLO = 1I = 0.082; GPT1 = 0.653; AcPA = 0.400; AcPB = 0.599; AcPC = 0.001; PGDA = 0.944; PGM1(1) = 0.906; EsD1 = 0.897; E2+ = 0.048; HpI = 0.394; GcI = 0,919; Tfc = 0.987; r(O) = 0.669; p(A) = 0.184; q(B) = 0.146; M = 0.711; Le = 0.411; P1+ = 0.521; t = 0.295. The genetic structure of Chukot Evens population is significantly nearer to that of the other ethnic groups of the North-East, in comparison with the genetic structure of Evenks of the Middle Siberia.
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PMID:[Genetic structure of the populations of native inhabitants in the northeastern USSR. V. The Chukot Evens]. 293 99

The enzyme pattern of 13 cases of malignant fibrous histiocytoma (MFH) and 11 cases of myxofibrosarcoma (MFS), a malignant myxomatous soft tissue tumor of fibroblastic histiocytic origin, has been studied. 6 of the 13 MFHs were analyzed enzyme histochemically at the light microscopic level and 7 on the ultrastructural level; of the 11 MFSs 9 were analyzed enzyme histochemically at the light microscopic level and 2 on the ultrastructural level. Differences were observed in the subjectively estimated enzyme activity between low grade MFS and high grade MFS and MFH, and also between histiocyte-like and fibroblast-like tumor cells. Generally a strong reaction of oxidoreductase enzymes (NADH2-diaphorase, NADPH2-diaphorase, glucose-6-phosphate dehydrogenase) and hydrolytic enzymes (acid phosphatase and leucine aminopeptidase) was found in the high grade tumors and was usually higher in the histiocyte-like than in the fibroblast-like cells. Ultrastructurally acid phosphatase occurred predominantly in primary and secondary lysosomes and Golgi zones of the histiocyte-like cells. A strong reaction of alkaline phosphatase was found light microscopically in 2 of 5 MFHs and 5 of 9 MFSs. Ultrastructurally alkaline phosphatase was located along the cytoplasmic membrane of predominantly fibroblast-like cells in 3 of 7 MFHs and 1 of 2 MFSs. The results agree with the concept of two main cell types in MFH and MFS, fibroblasts and histiocytes.
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PMID:Enzyme histochemistry of malignant fibroblastic histiocytic tumors. A light and electron microscopic analysis. 608 56

The genetic structure of three Asiatic eskimos subpopulations (402 individuals), five coast chuckchies subpopulations (1793 individuals) and three reindeer chuckchies subpopulations (559 individuals) have been studied for 26 electrophoretic protein systems (33 loci). These are: adenilate-kinase (AK), diaphorase NAD X H (Dia), glyoxalase-1 (GLO-1), glucose-6-phosphate dehydrogenase (6GPT), glutamatpyruvate transaminase (GPT), glutamicoxalate transaminase (GOT), carbonic anhydrase-1 (Ca-1), catalase (Ct), acid phosphatase (AcP), lactate dehydrogenase (loci LDH-A and LDH-B), leucine aminopeptidase (Lap), malatedehydrogenase (MDH), purine nucleoside phosphorylase (PNP), superoxide dismutase (Sod), 6-phosphogluconate dehydrogenase (PGD), phosphoglucomutase (loci PGM1 and PGM2), cholinesterase (loci c1--c5), alkaline phosphatase (Pp), esterase D (EsD), red cell esterase (Est) - 4 loci, albumin (Alb), haptoglobin (Hp), hemoglobine (Hb A and B), group-specific component (Gc), transferrin (Tf), ceruloplasmin (Cp). In addition, AB0 and Rh system blood groups and phenyl thiocarbamide taste sensitivity (PTC) have been studied. 12 of 36 loci are polymorphic (33.33%), heterozygosity for all loci in eskimos, coastal and reindeer chuckchies being 0.118 +/- 0.005, 0.130 +/- 0.002 and 0.120 +/- 0.004, respectively. These estimates do not differ essentially from heterozygosity at these loci for mongoloid groups living further south. The test for interpopulation heterogeneity has permitted to estimate contribution of the loci to the differentiation of these populations. The least heterogeneity has been found at loci where gene frequency distribution is the most specific for these ethnic groups.
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PMID:[Genetic structure of the populations of native inhabitants in the northeastern USSR. III. Asiatic Eskimos and the coast and reindeer Chukchi]. 643 3

The activities of NADH2-diaphorase, leucine aminopeptidase, and acid phosphatase were studied histochemically in spermatozoa and seminal plasma of 28 ejaculates from 13 men with proven fertility and in 31 ejaculates from 29 men living in infertile marriages. The enzyme activities were correlated with the spermiogram. The NADH2-diaphorase activity was located exclusively in the midpiece of the spermatozoa, while the activities of leucine aminopeptidase and acid phosphatase seemed to be located only on the surface. A positive correlation was found between the NADH2-diaphorase activity and the spermatozoal motility, density, and morphology.
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PMID:Enzyme histochemical studies of human spermatozoa correlated with the spermiogram. 689 11