Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.6.5.2 (
NQO1
)
6,196
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Individual variations in xenobiotic metabolism affect the sensitivity to diseases. In this study, the impacts of sex, age and race/ethnicity on drug-processing genes and NRF2 genes in human livers were examined via QuantiGene multiplex suspension array (226 samples) and qPCR (247 samples) to profile the expression of nuclear receptors, cytochrome P450s, conjugation enzymes, transporters, bile acid metabolism and NRF2-regulated genes. Sex differences were found in expression of about half of the genes, but in general the differences were not large. For example, females had higher transcript levels of
CAT, GCLC, HO-1, KEAP1, SOD1
, and
TXNRD1
compared to males via qPCR. There were no apparent differences due to age except children had higher
GCLM
and elderly had higher
MRP3
African Americans had lower expression of
FXR
but higher expression of
HO-1
, Caucasians had higher expression of
OAT2
, and Hispanics had higher expression of
FXR, SULT2A1, SHP
, and
BSEP
An examination of 34 diseased and control human liver samples showed that compared to disease-free livers, fibrotic livers had higher
NQO1
, GCLC, GCLM
and
NRF2
; hepatocellular carcinoma had higher transcript levels of
NQO1
and
KEAP1
, and steatotic livers had lower
GCLC
,
GCLM
and
HO-1
expression. In summary, in drug-processing gene and NRF2 genes, sex differences were the major findings, and there were no apparent age-differences and race/ethnicity differences occurred for a few genes. These descriptive findings could add to our understanding of the sex-, age-, and race/ethnicity -dependent differences in drug-processing genes, as well as NRF2 genes in normal and diseased human livers.
Significance Statement
Significance statement
In human liver drug-processing and NRF2 genes, sex differences were the main finding. There were no apparent differences due to age, except children had higher
GCLM
and elderly had higher
MRP3
African Americans had lower expression of
FXR
but higher expression of
HO-1
, Caucasians had higher expression of
OAT2
, and Hispanics had higher expression of
FXR, SHP, SULT2A1
and
BSEP
.
...
PMID:Sex-, age-, and race/ethnicity-dependent variations in drug-processing and NRF2-regulated genes in human livers. 3316 98
