Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.6.5.2 (NQO1)
 6,196 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Frozen sections of eight odontogenic cysts, including one keratocyst, were incubated to show the following enzyme activities: NADH2 diaphorase, NADPH2 diaphorase, glucose-6-phosphate dehydrogenase, acid phosphatase and leucine aminopeptidase. The disbribution of lipid was shown by the oil red 0 method. The activities of all three oxidative enzymes were strongest in epithelial cells bordering hyalin bodies and in basal cells in the epithelial lining. Hydrolytic enzyme activity was absent from all but the most superficial epithelial cells but was present in macrophages and, in lesser amounts, in granular material in the same sections. The granular material frequently contained lipid. The lack of hydrolytic enzyme activity in bordering epithelial cells is inconsistent with the theory that hyalin bodies form from degenerating blood vessels. High aerobic oxidative enzyme activity in the same cells also conflicts with the concept that the bodies are a keratinous product. The findings lend support to the theory that hyalin bodies are an epithelial secretion.
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PMID:Enzyme histochemical studies on the formation of hyalin bodies in the epithelium of odontogenic cysts. 5 1

Of a total of 1,420 odontogenic cysts, 52 (3.3%) were diagnosed as odontogenic keratocysts. Clinical and histological findings in these 52 cysts are reported. Frozen sections of 26 of the keratocysts were incubated to show the following enzyme activities: NADH2- and NADPH2-diaphorase, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, acid phosphatase, leucine aminopeptidase and ATPase. Furthermore, keratinization was studied with the rhodamine B method and lipids with the oil red O, the OTAN and the acid hematein methods. Sections from epidermis, oral mucosa, radicular cysts, residual cysts and follicular cysts served as reference material. The oxidative enzymes showed strong activity in the keratocyst epithelium which contrasted with weak activity in the reference cysts. Acid phosphatase activity was weak in all epithelia except that in keratocysts, which displayed a marked activity. In the fibrous capsule of the keratocyst a high activity of leucine aminopeptidase was recorded. This high activity contrasted with a weak activity in the reference material. The significance of the histochemical results in relation to the aggressive behavior of the keratocyst is discussed.
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PMID:Odontogenic keratocysts: a clinical and histological study with special reference to enzyme histochemistry. 14 97

The protective action of aspartic acid on isolated and perfused rat liver was studied. In case of D-galactosamine intoxication the GOT, GPT and SDH activity and the lactate and pyruvate concentration in the perfusion medium were less augmented and the glycogen level in hepatic tissue was less diminished in animals treated with aspartic acid, as compared to controls. The histochemical applied (PAS reaction for glycogen, nucleic acids, NADH2-diaphorase, glucose-6-phosphatase and membrane-ATP-ase), also stated a protecting effect in the treated animals. The protective action of aspartate is hypothetically considered to be exerted by its capacity to reestablish the cellular deficit of pyridine nucleotides and thus to improve the synthesis of nucleic acids, glycoprotein and glycolipids or/and by its participation in various metabolic pathways.
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PMID:Protecting action of aspartate on the hepatic changes induced by D-galactosamine. 18 87

The hepatoprotective action of Silymarin was studied in 65 male Wistar rats, prior to and following D-galactosamine intoxication. There was a marked reduction in the histological and ultrastructural changes in the nucleolus, nuclear membrane, mitochondria, granular and agranular endoplasmic reticulum and lysosomes of the liver cell and also in the Kupffer stellate cells. The reduction in glycogen and RNA loss was determined biochemically. The activities of many enzymes were kept constant (oxidoreductases, NADH2 diaphorase, G-6-phosphatase, Mg++ and K+/Na+-dependent ATPases, acid phosphatases).
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PMID:[The action of silymarin on Galactosamine-induced hepatitis in the rat (author's transl)]. 18 15

One hour after a single i.v. dose of 250 mg/kg folic acid, the straight portion of distal tubules in the outer medulla of rat kidneys showed a distinct reduction in succinate dehydrogenase, NADH2-diaphorase, glutamate dehydrogenase, cytochrome oxydase, Na+/K+-ATPase, and acid phosphatase activity. In contrast, the proximal tubules exhibited only a reduction in glutamate dehydrogenase and alkaline phosphatase activity. At this time the straight portion of the distal tubules, whose enzyme activity had changed, showed partly regressive epithelial changes. 24 hours after folic acid administration an even greater reduction in enzyme activity had occurred in the straight portion of distal tubules; these structures also became dilated. The adjacent collecting tubules and the corresponding proximal tubules were also severely dilated, the proximal tubules showing a loss in enzyme acitivities similar to those observed in the distal tubules. 48 hours after folic acid administration the changes largely resembled those observed after 24 hours, but were more pronounced. At this time a tubular regeneration was observed. 72 hours after folic administration extensive normalization of the histological and histochemical changes had occured. It is postulated that a disturbance of the hairpin counter-current mechanism occurs as a result of a direct, concentration-dependent effect of folic acid on the enzymes of the energy supplying metabolism. A dilation in the region of the loop of Henle and the collecting tubules occurs subsequently.
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PMID:Enzyme histochemistry of rat folic acid nephropathy. 19 86

Histochemical and biochemical studies on the folate metabolism (folic acid an its principal enzyme-dihydrofolate-reductase) in bovine cortex - gyrus marginallis in the process of ageing were performed, in parallel with NADH2-cytocrom-C-reductase (diaphorase). Folic acid and folate enzyme, weak positive in neurons in young age, increased in old age in nerve cells and especially in their processes and in capillaries. The diaphorase strongly increased in all cells, glia and vessels, in old age.
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PMID:Histochemical study on the dihydrofolatereductase and folic acid in mammalian brain cortex. 54 85

Comparative analyses of the fibre content (FG, FOG, and SO fibres) and the capillary density (the number of capillaries surrounding individual fibres and the capillary/fibre ratio) were performed in hind limb muscles of the cat. Cross-sections from the tenuissimus, the biceps femoris, the lateral head (LG) and the medial head (MG) of the gastrocnemius and the soleus were cut in a cryostat. The sections were stained histochemically for the NADH2-diaphorase and alkaline (pH 9.4) actomyosin ATPase activity, which enables differentiation of different types of fibres. The endothelium of the capillaries was identified via staining for unspecific alkaline ATPase activity. The number of capillaries surrounding each individual muscle fibre had a positive correlation, first to the oxidative capacity and secondly to the average diameter of the fibres. The thin tenuissimus muscle did not differ in this respect from the thicker muscles. The highest proportion of SO fibres was found in the soleus and the MG muscles. FG fibres of two different types were dominating the fibre mass in the biceps femoris and the LG muscles, while the tenuissimus contained more FOG fibres than these muscles. In general the FG fibres had a larger diameter than the FOG and the SO fibres. The soleus and the MG muscles contained larger fibres than the other examined muscles. FG fibres were surrounded by fewer capillaries than FOG and SO fibres. The soleus and the MG muscles, with a higher percentage of SO fibres and also larger fibres, had the largest number of capillaries around the fibres and the highest capillary/fibre ratio.
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PMID:Capillary supply of the muscle fibre population in hindlimb muscles of the cat. 66 57

Leukocytes, approached by histoenzymological methods for demonstration of dihydrofolate dehydrogenase, NADH2-diaphorase, lactate dehydrogenase and alcaline phosphatase activities, provided information about the impaired metabolic balance of thyrotoxicosis, Cushing and Addison diseases. The most relevant variations were found in thyrotoxicosis, the most sensitive enzyme was dihydrofolate dehydrogenase and the less sensitive was alcaline phosphatase. The neutrophils and lymphocytes had more evident enzymic variations.
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PMID:Cytoenzymologic activities of some oxidroeductases and alkaline phosphatase of leucocytes in Basedow, Cushing and Addison diseases. 81 Oct 42

The activity of succinatedehydrogenase, mitochondrial alpha-glycerophosphatedehydrogenase and NADH2-diaphorase was studied cytochemically and histochemically in the myocardium and lymphocytes of rats and mice after the effect of hypoxia in a hyperbaric chamber. A short-duration effect of hypoxia and step-wise adaptation during a month was shown to result in a growing activity of the enzymes both in the lymphocytes and in the myocardium. Hypoxia during a month and readaptation during the same period caused a monotonous decrease of the activity of dehydrogenases. The conducted analysis permitted to reveal a distinct correlation between the activity of dehydrogenases in the lymphocytes and in the myocardium. The authors emphasize the similarity of the changes in the activity of the enzymes in the lymphocytes and in the myocardium.
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PMID:[Activity of the dehydrogenases of the myocardium and lymphocytes in experimental hypoxia]. 115 23

By Coxsackie virus type B3 subconjunctival administration it has been noticed the appearance of a antiviral defense mechanism, revealed by the presence in conjunctival chorion of lymphocytes, plasmocytes and active macrophages. It had also been studied by histochemical methods the responsive way at conjunctival level of NADH2-cytocrom-C-reductase (diaphorase) in experimentally obtained viral aggression. The injected virus has determined the stimulation of the activity of this enzyme at epithelial level, which play an important role in mucous conjunctival protection. The lymphocytes appearing in a great number presented an intense enzymatic reaction in chorion, which denotes a living synthetic and secretory metabolical activity.
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PMID:[Diaphorase activity at the conjunctival level in an experimentally produced viral infection]. 132 35


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