Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.6.5.2 (
NQO1
)
6,196
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Rhododendrol (RD) is a potent tyrosinase inhibitor that is metabolized to RD-quinone by tyrosinase, which may underlie the cytotoxicity of RD and
leukoderma
of the skin that may result. We have examined how forced expression of the NAD(P)H quinone dehydrogenase, quinone 1 (
NQO1
), a major quinone-reducing enzyme in cytosol, affects the survival of RD-treated cells. We found that treatment of the mouse melanoma cell line B16BL6 or normal human melanocytes with carnosic acid, a transcriptional inducer of the
NQO1
gene, notably suppressed the cell killing effect of RD. This effect was mostly abolished by ES936, a highly specific
NQO1
inhibitor. Moreover, conditional overexpression of the human
NQO1
transgene in B16BL6 led to an expression-dependent increase of cell survival after RD treatment. Our results suggest that
NQO1
attenuates the cytotoxicity of RD and/or its metabolites.
...
PMID:NAD(P)H dehydrogenase, quinone 1 (NQO1), protects melanin-producing cells from cytotoxicity of rhododendrol. 2684 26
