Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.6.5.2 (
NQO1
)
6,196
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
NAD(P)H:quinone oxidoreductase 1 (
NQO1
;
DT-diaphorase
; DTD) is a cytosolic two-electron reductase, and compounds of the quinone family such as mitomycin C are efficiently bioactivated by this enzyme. The observation that
DT-diaphorase
is highly expressed in many cancerous tissues compared to normal tissues has provided us with a potentially selective target that can be exploited in the design of novel anticancer agents. Because of the relative lack of information about the cell-specific expression of
DT-diaphorase
, the purpose of this study was to map the distribution of this enzyme in normal human tissues. Fifteen tissue samples from normal human kidney were analyzed for expression of
DT-diaphorase
by immunohistochemistry (two-step indirect method). We found a specific high expression of
DT-diaphorase
in glomerular visceral epithelial cells (podocytes). These results suggest that a high expression of
DT-diaphorase
in podocytes could play a major role in the pathogenesis of renal toxicity and mitomycin C-induced hemolytic uremic syndrome, in which injury to the glomerular filtration mechanism is the primary damage, leading to a cascade of deleterious events including microangiopathic hemolytic anemia and
thrombocytopenia
. This observation has potential therapeutic implications because the
DT-diaphorase
metabolic pathway is influenced by many agents, including drugs, diet, and environmental cell factors such as pH and oxygen tension.
...
PMID:NAD(P)H: quinone oxidoreductase 1 expression in kidney podocytes. 1258 57
