Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.6.5.2 (
NQO1
)
6,196
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The levels and subcellular distribution of enzymes involved in defenses against reactive oxygen superoxide dismutase (SOD; E.C.1.15.1.1), glutathione peroxidase (GPX; E.C.1.11.1.9), catalase (CAT; E.C.1.11.1.6), and
DT-diaphorase
(DT; E.C.1.6.99.2) and of the conjugating enzymes glutathione transferase (GST; E.C.2.5.1.18) and p-sulphotransferase (p-ST; E.C.2.8.2.1) in the corpus luteum of ovaries from pregnant and non-pregnant pigs were investigated. In addition, non-protein thiols and glutathione reductase (
GRD
; E.C.1.6.4.2) were examined in the same manner. The total cytosolic activities of CAT, DT,
GRD
, and p-ST were significantly increased, whereas total GST activity was decreased in the pregnant corpus luteum compared to the corresponding activities in non-pregnant corpus luteum. In the case of the mitochondrial fraction from pregnant corpus luteum, GPX and
GRD
displayed significant increases in specific activity. Upon subfractionation of the mitochondrial fraction (i.e. mitoplast preparation), SOD activity was distributed equally between the mitoplasts and the supernatant. CAT and GPX activities were mainly recovered in the supernatant, while the major
GRD
activity pelleted with the mitoplasts. Microsomes from pregnant corpus luteum demonstrated increased specific GPX activity and decreased SOD activity compared to the non-pregnant corpus luteum. No differences in the non-protein thiol levels in the cytosolic, mitochondrial, or microsomal fractions from the corpus luteum were observed between non-pregnant and pregnant sows.
...
PMID:Levels and subcellular distributions of detoxifying enzymes in the ovarian corpus luteum of the pregnant and non-pregnant pig. 1048 30
Graves
' orbitopathy (GO) is a sight-threatening ocular disease that occurs in patients with hyperthyroidism and is especially associated with oxidative stress. Polydatin (PD) is a major active component of Polygonum cuspidatum Sieb. et Zucc. It has various therapeutic effects including anti-inflammatory and antioxidant properties. In the present study, we investigated the effects of PD on H
2
O
2
-induced oxidative stress in orbital fibroblasts in vitro and in a GO mouse model of orbital oxidative stress in vivo. The mechanisms responsible for these effects were investigated using standard molecular techniques. Our initial findings in GO mice were that PD attenuated orbital muscle adipose tissue expansion and lipid droplet accumulation through a nuclear factor E2-related factor 2 (NRF2)-mediated oxidative stress response involving the Keap1/Nrf2/ARE pathway. The results demonstrated that PD could reverse the accumulation of lipid droplets and production of reactive oxygen species (ROS) induced by H
2
O
2
and increase the expression of antioxidant genes such as NAD(P)H dehydrogenase, quinone 1 (
NQO1
).
NQO1
levels were the lowest in the GO mouse model. In addition, PD enhanced NRF2 nuclear translocation in cultured orbital fibroblasts. We also found that silencing NRF2, using RNA interference, reduced the protective effects of PD against H
2
O
2
-induced oxidative stress in orbital fibroblasts in vitro. Taken together, our results indicate that PD can reduce the production of ROS and inhibit adipogenesis in orbital fibroblasts in vitro and in vivo.
...
PMID:Polydatin attenuates orbital oxidative stress in Graves' orbitopathy through the NRF2 pathway. 3170 58
