Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.6.3.1 (
NADPH oxidase
)
11,281
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
PqqC
/D converts the biosynthetic intermediate purified from a pqqC mutant to pyrroloquinoline quinone (PQQ), and both NAD(P)H and cytosolic fraction, named as activating factor (ActF), are required to show its higher production. Dithiothreitol alone, as well as ActF plus NAD(P)H, enhanced the PQQ production by
PqqC
/D. Thioredoxin-thioredoxin reductase system with NADPH showed similar effect.
PqqC
/D made a tight complex with PQQ, however, in the presence of dithiothreitol, PQQ was dissociated from the protein. ActF showed
NADPH oxidase
activity which was enhanced by the addition of PQQ. These data suggest that
PqqC
/D produces the reduced PQQ from the intermediate in vivo, but in vitro, it is further oxidized by molecular oxygen and then the oxidized PQQ is trapped in
PqqC
/D to show product inhibition.
...
PMID:Factors required for the catalytic reaction of PqqC/D which produces pyrroloquinoline quinone. 1722 81
