Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.5.1.3 (
dihydrofolate reductase
)
5,819
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Incorporation of non-natural amino acids into proteins in vivo expands the scope of protein synthesis and design. p-
Acetylphenylalanine
was incorporated into recombinant
dihydrofolate reductase
(
DHFR
) in Escherichia coli via a computationally designed mutant form of the phenylalanyl-tRNA synthetase of the host.
DHFR
outfitted with ketone functionality can be chemoselectively ligated with hydrazide reagents under mild conditions.
...
PMID:A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins. 1201 34
