Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.5.1.3 (
dihydrofolate reductase
)
5,819
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The uptake of
glycolate oxidase
into peroxisomes has been studied using an in vitro import system. Import of
glycolate oxidase
was found to be ATP-dependent and temperature-dependent and specific for glyoxysomes. In these respects it resembles the import of isocitrate lyase into both glyoxysomes and leaf-type peroxisomes; thus the ATP-dependence and temperature dependence appear to be general properties of plant microbody protein import. Two mutant versions of
glycolate oxidase
were prepared lacking 59 amino acids of the N-terminus and 53 amino acids of C-terminus, respectively. Both were capable of ATP-dependent import, whereas a fusion protein consisting of the cytosolic protein
dihydrofolate reductase
linked to the last 20 amino acids of
glycolate oxidase
bound to glyoxysomes but did not enter the organelle.
...
PMID:Investigation of the energy requirement and targeting signal for the import of glycolate oxidase into glyoxysomes. 760 Oct 95
