Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.5.1.3 (
dihydrofolate reductase
)
5,819
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Newly synthesized proteins are usually exported through the endoplasmic reticulum (ER) and Golgi due to the presence in their primary sequence of a hydrophobic signal peptide that is recognized by the ER translocation system. However, some secreted proteins lack a signal peptide and are exported independently of ER-Golgi. Fibroblast growth factor (FGF)1 is included in this group of polypeptides, as well as S100A13 that is a small calcium-binding protein critical for
FGF1
export. Classically secreted proteins are transported into ER in their unfolded states. To determine the role of protein tertiary structure in
FGF1
export through the cell membrane, we produced the chimeras of
FGF1
and S100A13 with
dihydrofolate reductase
(
DHFR
). The specific
DHFR
inhibitor, aminopterin, prevents its unfolding. We found that aminopterin did not inhibit the release of
FGF1
:
DHFR
and S100A13:
DHFR
. Thus,
FGF1
and S100A13 can be exported in folded conformation.
...
PMID:Protein folding does not prevent the nonclassical export of FGF1 and S100A13. 1923 22
