Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.5.1.3 (
dihydrofolate reductase
)
5,819
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The local fluorescence probes, 2-(p-toluidino)-6-naphthalenesulfonic acid (
TNS
) and NADPH were employed to detect urea-induced conformation changes at each active site of
dihydrofolate reductase
(
DHFR
), respectively. The results indicate that local conformation change at DHF/
TNS
could be superimposed by the conformation change calculated from the enzyme activity change with a three-state model; while at NADPH site it is lagged in the first transition. This difference is further supported by the different relative changes of Michaelis constants at 0, 1 and 1.8 M urea for each substrate. Our results suggest that local conformation at DHF site is more flexible than that at NADPH site, and the urea-induced unfolding could be ascribed to a four-state transition.
...
PMID:Sequential conformation changes of chinese hamster ovary dihydrofolate reductase at its active sites. 1602 62
